High-Performance Liquid Chromatography Method for the Monitoring of the Allium Derivative Propyl Propane Thiosulfonate Used as Natural Additive in Animal Feed
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A new simple analytical method for monitoring propyl propane thiosulfonate (PTSO) in animal feed is presented. PTSO is an active ingredient from Allium spp. (like onion and shallot), proposed as a natural additive for feed being an efficient alternative to antibiotics used as growth promoter due to its efficiency improving animal health. Reversed-phase liquid chromatography with UV detection has been used and a previous sample treatment based on solid-liquid extraction has been developed and optimized in order to extract PTSO from a feed for laying hens using acetone as extraction solvent. The method has been characterized obtaining limits of detection and quantification of 11.2 and 37.3 mg kg−1, respectively, which are lower than the concentrations expected in samples containing this additive. The intra- and interday relative standard deviations (RSDs) were lower than 8.3 % in all the cases, and recoveries varied from 90.2 to 94.6 %. Finally, in order to check the unequivocal identification of PTSO, mass spectrometry detection was applied. The proposed method is a simple procedure for monitoring PTSO in commercial feed, being possible to implement it in routine laboratories for quantification purposes and stability studies of the distributed products.
KeywordsAllium extract Propyl propane thiosulfonate Natural growth promoter Feed HPLC
The authors are thankful for the financial support from the Campus of International Excellence – BioTic Granada (Project CEI2013-MP-27). NAM thanks the Junta the Andalucía for a postdoctoral contract.
Conflict of Interest
Paloma Abad declares that he has no conflict of interest. Francisco J. Lara declares that he has no conflict of interest. Natalia Arroyo-Manzanares declares that he has no conflict of interest. Alberto Baños declares that he has no conflict of interest. Enrique Guillamón declares that she has no conflict of interest. Ana M. García-Campaña declares that she has no conflict of interest. This article does not contain any studies with human or animal subjects.
- Blumenthal M, Goldberg A, Brinckmann J (2000) Herbal medicine: expanded commission E monographs. Integrative Medicine Communications, NewtonGoogle Scholar
- Cavallito C, Bailey J (1944) Allicin, the antibacterial principle of Allium sativum. Isolation, physical properties and antibacterial action. J Am Chem Soc 66:1944–1952Google Scholar
- Costa L, Panhoza MT, Miyada VS (2007) Herbal extracts as alternatives to antimicrobial growth for weanling pigs. Braz J Anim Sci 36:589–595Google Scholar
- European Commission (2003) Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition. Official Journal of the European Union L262 (18.10.2003)Google Scholar
- Friedman M, Henika PR, Mandrell RE (2002) Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. J Food Prot 65:1545–1560Google Scholar
- Kim DK, Lillehoj HS, Lee SH, Lillehoj EP, Bravo D (2012) Improved resistance to Eimeria acervulina infection in chickens due to dietary supplementation with garlic metabolites. Br J Nutr 109:1–13Google Scholar
- Liu G et al (2008) Effects of herbal extract supplementation on growth performance and insulin-like growth factor (IGF)-I system in finishing pigs. J Anim Feed Sci 17:538–547Google Scholar
- Oetting L et al (2006) Effects of herbal extracts and antimicrobials on apparent digestibility, performance, organs morphometry and intestinal histology of weanling pigs. Braz J Anim Sci 35:1389–1397Google Scholar
- Utiyama CE, Oetting LL, Giani PA, Ruiz UDS, Miyada VS (2006) Effects of antimicrobials, prebiotics, probiotics and herbal extracts on intestinal microbiology, diarrhea incidence and performance of weanling pigs. Braz J Anim Sci 35:2357–2359Google Scholar