Food Analytical Methods

, Volume 7, Issue 1, pp 224–233 | Cite as

Molecular Identification of Four Genetically Modified Maize (Bt11, Bt176, Mon810 and T25) by Duplex Quantitative Real-Time PCR

  • Maher Chaouachi
  • Mohamed Salem Zellama
  • Nesrine Nabi
  • Ahmed Ben Hafsa
  • Khaled Saïd
Article
First Online:
Received:
Accepted:

Abstract

In response to the increasing number of genetically modified (GM) events released on the market, control laboratories explore various strategies to simplify and reduce the number of tests needed to characterise the content in genetically modified organism (GMO) of a given sample. Lastly, multiplexing is considered as one of the possible ways to decrease the time and cost of analysis. Here, we report the development of four duplex polymerase chain reaction (PCR) tests for the identification and the quantification of four maize transformation events from which commercial lines have been authorised in Europe namely, Bt11 and Bt176 (Syngenta, DE, USA), Mon810 MaisGard™ (Monsanto, MO, USA) and T25 Liberty Link™ (Bayer CropScience, Monheim, Germany). The duplex PCR tests combine a maize-specific PCR test hybridising in the Adh1 locus with an event-specific detection system designed on a junction fragment for each of these four GM maize. Real-time PCR tests, suitable to comply with the European regulation, were designed by using Taqman® chemistry.

Keywords

Maize GMO Event-specific Quantification Duplex QRT-PCR 
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Notes

Conflict of Interest

All the authors have no financial relationship with the organisation that sponsored the research. The Conflict of Interest statements must list each author separately by name:

Dr. Maher Chaouachi declares that he has no conflict of interest. Mr. Mohamed Salem Zellama declares that he has no conflict of interest. Ms. Nesrine Nabi declares that he has no conflict of interest. Mr. Ahmed Ben Hafsa declares that he has no conflict of interest. Pr. Khaled Said declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Maher Chaouachi
    • 1
  • Mohamed Salem Zellama
    • 1
  • Nesrine Nabi
    • 1
  • Ahmed Ben Hafsa
    • 1
  • Khaled Saïd
    • 1
  1. 1.Laboratoire de Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieur de Biotechnologie de MonastirUniversité de MonastirMonastirTunisia

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