Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Comparative Study of Monoclonal and Recombinant Antibody-Based Immunoassays for Fungicide Analysis in Fruit Juices

Abstract

A comparative study of the analytical performance of enzyme-linked immunosorbent assays (ELISAs), based on monoclonal and recombinant antibodies, for the determination of fungicide residues in fruit juices has been carried out. To this aim, three murine hybridoma cell lines secreting specific monoclonal antibodies against (RS)-2-(2,4-dichlorophenyl)-3-(1H-1,2,4-triazol-1-yl)propyl-1,1,2,2-tetrafluoroethyl ether (tetraconazole), 2-(4-triazolyl)benzimidazole (thiabendazole), and (RS)-1-(β-allyloxy-2,4-dichlorophenylethyl)imidazole (imazalil) were used as a source of immunoglobulin gene fragments for the production of single-chain variable fragment (scFv) and fusion scFv-pIII recombinant antibodies in Escherichia coli. Selected recombinant antibodies displayed cross-reactivity profiles very similar to those of the parent monoclonal antibodies. Imazalil and tetraconazole recombinant antibodies showed one order of magnitude lower affinity than their respective monoclonal antibodies, whereas the thiabendazole recombinant antibodies showed an affinity similar to that of their parent monoclonal antibody. On the other hand, scFv-pIII fusion fragments showed similar analytical properties as, and occasionally better than, scFv recombinant antibodies. Finally, ELISAs developed from each antibody type showed similar analytical performance when applied to the analysis of the target fungicides in fruit juices.

This is a preview of subscription content, log in to check access.

Fig. 1

References

  1. Abad A, Manclús JJ, Moreno M, Montoya A (2001) J AOAC Int 84:1–6

  2. Alcocer MJC, Doyen C, Lee HA, Morgan MRA (2000) J Agric Food Chem 48:4053–4059

  3. Brichta J, Vesela H, Franek M (2003) Vet Med 48:237–247

  4. Brichta J, Hnilova M, Viskovic T (2005) Vet Med 50:231–252

  5. Charlton K, Harris WJ, Potter AJ (2001) Biosens Bioelec 16:639–646

  6. EU Pesticide Database (2013) Pesticide EU-MRLs. http://ec.europa.eu/sanco_pesticides/public/index.cfm. Accessed Jan 2013

  7. Ferrer C, Martínez-Bueno MJ, Lozano A, Fernández-Alba AR (2011) Talanta 83:1552–1561

  8. Garret SD, Appleford DJA, Wyatt GM, Lee HA, Morgan MRA (1997) J Agric Food Chem 45:4183–4189

  9. Graham BM, Porter AJ, Harris WJ (1995) J Chem Technol Biotech 63:279–289

  10. Hiemstra M, de Kok A (2007) J Chromatog A 1154:3–25

  11. Kipriyanov SM, Moldenhauer G, Little M (1997) J Immunol Meth 200:69–77

  12. Kramer K, Hock B (2007) Recombinant antibodies for agrochemicals: Evolutionary optimization. In: Kennedy IR, Solomon KR, Gee SJ, Crossan AN, Wang S, Sánchez-Bayo F (eds) Rational environmental management of agrochemicals: Risk assessment, monitoring, and remedial action. ACS Symposium Series, vol. 966, pp 155−170

  13. Krebber A, Bornhauser S, Burmester J, Honegger A, Willuda J, Bosshard HR, Plückthun A (1997) J Immunol Meth 201:35–55

  14. Leong SSJ, Chen WN (2008) Chem Engin Sci 63:1401–1414

  15. Li T, Zhang Q, Liu Y, Chen D, Hu B, Blake DA, Liu F (2006) J Agric Food Chem 54:9085–9091

  16. Manclús JJ, Moreno M, Plana E, Montoya A (2008) J Agric Food Chem 56:8790–8800

  17. Markus V, Janne L, Urpo L (2011) Trends Anal Chem 30:219–226

  18. Mersmann M, Schmidt A, Tesar M, Schöneberg A, Welschof M, Kipriyanov S, Terness P, Little M, Pfizenmaier K, Moosmayer D (1998) J Immunol Meth 220:51–58

  19. Moreno M, Plana E, Montoya A, Caputo P, Manclús JJ (2007) Food Addit Contam 24:704–712

  20. Morozova VS, Levashova AI, Eremin SA (2005) J Anal Chem 60:202–217

  21. Nishi K, Imajuku Y, Nakata M, Ohde K, Miyake S, Morimune K, Kawata M, Ohkawa H (2003) J Pest Sci 28:301–309

  22. Nishi K, Ishiuchi M, Morimune K, Ohkawa H (2005) J Agric Food Chem 53:5096–5104

  23. Scholthof KB, Whang G, Karu AE (1997) J Agric Food Chem 45:1509–1517

  24. Sheedy C, MacKenzie CR, Hall JC (2007) Biotech Adv 25:25333–25352

  25. Tout NL, Yau KYF, Trevors JT, Lee H, Hall JC (2001) J Agric Food Chem 49:3628–3637

  26. Webb SR, Lee H, Hall JC (1997) J Agric Food Chem 45:535–541

  27. Yau KYF, Tout NL, Trevors JT, Lee H, Hall JC (1998) J Agric Food Chem 46:4457–4463

  28. Yoshioka N, Akiyama Y, Matsuoka T, Mitsuhashi T (2010) Food Control 21:212–216

Download references

Acknowledgments

This work was funded by Ministerio de Educación y Ciencia (MEC, Spain, Project AGL2002-03266). E. P. was the recipient of a doctoral fellowship from Conselleria d’Educació (Generalitat Valenciana, Spain).

Conflict of Interest

María-José Moreno, Emma Plana, Juan J. Manclús, and Angel Montoya declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects.

Author information

Correspondence to Angel Montoya.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Moreno, M., Plana, E., Manclús, J.J. et al. Comparative Study of Monoclonal and Recombinant Antibody-Based Immunoassays for Fungicide Analysis in Fruit Juices. Food Anal. Methods 7, 481–489 (2014). https://doi.org/10.1007/s12161-013-9655-z

Download citation

Keywords

  • Fungicide residues
  • Monoclonal antibodies
  • Recombinant antibodies
  • Immunoanalysis
  • ELISA
  • Fruit juices