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Food Analytical Methods

, Volume 6, Issue 4, pp 1024–1032 | Cite as

Validation of an HPLC Methodology for the Identification and Quantification of Biogenic Amines in Chicken Meat

  • César A. Lázaro
  • Carlos A. Conte-Júnior
  • Fernanda L. Cunha
  • Eliane T. Mársico
  • Sérgio B. Mano
  • Robson M. Franco
Article

Abstract

This study validated a high performance liquid chromatography (HPLC) method to determine biogenic amines in chicken meat. For the identification of biogenic amines, an isocratic elution system coupled with a UV detector (254 nm) was used after a perchloric acid (5 %) extraction and benzoyl chloride derivatization of the samples. The standards of tyramine, putrescine, cadaverine, spermidine, and spermine were used for the following validation parameters: selectivity, linearity, accuracy, recovery, limit of detection and quantification, and robustness. Finally, chicken meat commercialized in two types of packaging was evaluated. The results showed an excellent selectivity and separation of all amines, r 2 > 0.99, relative standard deviation <5 %, recovery between 64 and 112 %, limits of detection and quantification between 0.03–1.25 and 0.15–5.00 μg L−1, respectively, and appropriate robustness for the proposed methodology. Moreover, both chicken meat commercial packages had similar values for all amines; only tyramine was significantly different (P ≤ 0.05). The proposed method was suitable to detection and quantification of simultaneous five biogenic amines in chicken meat.

Keywords

Validation HPLC Biogenic amines Meat Chicken 

Notes

Acknowledgments

César Lázaro acknowledges the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior for the scholarship Programa Estudante Convenio Pos-Graduação.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • César A. Lázaro
    • 1
  • Carlos A. Conte-Júnior
    • 2
  • Fernanda L. Cunha
    • 1
  • Eliane T. Mársico
    • 2
  • Sérgio B. Mano
    • 2
  • Robson M. Franco
    • 2
  1. 1.Postgraduate Program in Veterinary MedicineUniversidade Federal FluminenseNiteróiBrazil
  2. 2.Department of Food Technology, Faculty of VeterinaryUniversidade Federal FluminenseNiteróiBrazil

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