Food Analytical Methods

, Volume 5, Issue 1, pp 1–7

Multicenter Collaborative Trial Evaluation of a Method for Detection of Human Adenoviruses in Berry Fruit

  • Martin D’Agostino
  • Nigel Cook
  • Ilaria Di Bartolo
  • Franco M. Ruggeri
  • Alessandra Berto
  • Francesca Martelli
  • Malcolm Banks
  • Petra Vasickova
  • Petr Kralik
  • Ivo Pavlik
  • Petros Kokkinos
  • Apostolos Vantarakis
  • Kirsi Söderberg
  • Leena Maunula
  • Katharina Verhaelen
  • Saskia Rutjes
  • Ana Maria de Roda Husman
  • Renate Hakze
  • Wim Van der Poel
  • Agnieszka Kaupke
  • Iwona Kozyra
  • Artur Rzeżutka
  • Jasna Prodanov
  • Sava Lazic
  • Tamas Petrovic
  • Anna Carratala
  • Rosina Gironés
  • Marta Diez-Valcarce
  • Marta Hernandez
  • David Rodriguez-Lazaro
Article

DOI: 10.1007/s12161-011-9287-0

Cite this article as:
D’Agostino, M., Cook, N., Di Bartolo, I. et al. Food Anal. Methods (2012) 5: 1. doi:10.1007/s12161-011-9287-0

Abstract

The qualitative performance characteristics of a qPCR-based method to detect human adenoviruses in raspberries were determined through a collaborative trial involving 11 European laboratories. The method incorporated a sample process control (murine norovirus) and an internal amplification control. Trial sensitivity or correct identification of 25-g raspberry samples artificially contaminated with between 5 × 102 and 5 × 104 PFU was 98.5%; the accordance and concordance were 97.0%. The positive predictive value was 94.2%. The trial specificity or percentage correct identification of non-artificially contaminated samples was 69.7%; the accordance was 80.0% and the concordance was 61.7%. The negative predictive value was 100%. Application of a method for the detection of human adenoviruses in food samples could be useful for routine monitoring for food safety management. It would help to determine if a route of contamination exists from human source to food supply chain which pathogenic viruses such as norovirus and hepatitis A virus could follow.

Keywords

Human adenovirus Multicenter ring trial Detection 

Copyright information

© British Crown Copyright as represented by Fera 2011

Authors and Affiliations

  • Martin D’Agostino
    • 1
  • Nigel Cook
    • 1
  • Ilaria Di Bartolo
    • 2
  • Franco M. Ruggeri
    • 2
  • Alessandra Berto
    • 3
  • Francesca Martelli
    • 3
  • Malcolm Banks
    • 3
  • Petra Vasickova
    • 4
  • Petr Kralik
    • 4
  • Ivo Pavlik
    • 4
  • Petros Kokkinos
    • 5
  • Apostolos Vantarakis
    • 5
  • Kirsi Söderberg
    • 6
  • Leena Maunula
    • 6
  • Katharina Verhaelen
    • 7
  • Saskia Rutjes
    • 7
  • Ana Maria de Roda Husman
    • 7
  • Renate Hakze
    • 8
  • Wim Van der Poel
    • 8
  • Agnieszka Kaupke
    • 9
  • Iwona Kozyra
    • 9
  • Artur Rzeżutka
    • 9
  • Jasna Prodanov
    • 10
  • Sava Lazic
    • 10
  • Tamas Petrovic
    • 10
  • Anna Carratala
    • 11
  • Rosina Gironés
    • 11
  • Marta Diez-Valcarce
    • 12
  • Marta Hernandez
    • 12
  • David Rodriguez-Lazaro
    • 12
  1. 1.Food and Environment Research Agency (FERA)YorkUK
  2. 2.Istituto Superiore di SanitaRomeItaly
  3. 3.Animal Health Veterinary Laboratories AgencyWeybridgeUK
  4. 4.Veterinary Research InstituteBrnoCzech Republic
  5. 5.University of PatrasPatrasGreece
  6. 6.University of HelsinkiHelsinkiFinland
  7. 7.National Institute for Public Health and the Environment (RIVM)Bilthoventhe Netherlands
  8. 8.Wageningen University ResearchLelystadthe Netherlands
  9. 9.National Veterinary Research InstitutePulawyPoland
  10. 10.Scientific Veterinary Institute Novi-SadNovi SadSerbia
  11. 11.University of BarcelonaBarcelonaSpain
  12. 12.Instituto Tecnológico Agrario de Castilla y León (ITACyL)ValladolidSpain

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