Food Analytical Methods

, Volume 5, Issue 3, pp 332–338 | Cite as

Optimization of Conditions for the Extraction of Antioxidants from Leaves of Syzygium cumini L. Using Different Solvents

  • Sumitra V. ChandaEmail author
  • Mital J. Kaneria


The aim of the present study was to find out the best method for extracting antioxidants from Syzygium cumini L. leaves. The extraction was done by three different methods: sequential cold percolation extraction method, decoction extraction method, and maceration extraction method. Antioxidant activity, total phenol, and flavonoid content were determined in all different extracts of various extraction methods of S. cumini L. leaves. Antioxidant activity was tested by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical, superoxide anion radical and hydroxyl radical scavenging activities, and reducing capacity assessment. Sequential cold percolation extraction method proved to be the best extraction method. The acetone extract had maximum phenol and flavonoid content and showed best DPPH free radical scavenging activity and reducing capacity assessment. Ethyl acetate extract showed best superoxide radical scavenging activity, while aqueous extract showed best hydroxyl radical scavenging activity. It can be concluded that sequential cold percolation extraction method is the best method of extracting leaf antioxidants for this plant at least.


Syzygium cumini Antioxidant activity Extraction methods Total phenol content DPPH 



The authors thank Prof. S.P. Singh, Head, Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India for providing excellent research facilities. One of the authors, Mr. Mital Kaneria, is thankful to University Grants Commission, New Delhi, India for providing financial support as Junior Research Fellow.


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© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Phytochemical, Pharmacological and Microbiological Laboratory, Department of BiosciencesSaurashtra UniversityRajkotIndia

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