Detection of Adulteration of Meat and Meat Products with Buffalo Meat Employing Polymerase Chain Reaction Assay
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The primer pair was designed based on mitochondrial d-loop gene for detection of adulteration of buffalo meat in admixed meat and meat products by polymerase chain reaction (PCR) assay. Amplification of 537-bp DNA fragments was observed from buffalo, without any cross-reaction with cattle, sheep, goat, pig, and chicken. The amplification was further confirmed by BamHI restriction enzymes. No adverse effect of processing was found on PCR amplification of buffalo meat DNA extracted from processed meat and meat products, even from meat emulsion autoclaved at 121 °C, 20 psi for 15–20 min. The detection limit for buffalo meat was found to be 1% in the admixed meat and meat products; however, very faint and inconsistent results were obtained in autoclaved meat emulsion at 1% level. The developed PCR assay was found to be specific for buffalo and could be a useful tool for detection of meat adulteration.
KeywordsMeat species Adulteration Detection Processed meat products Species-specific PCR assay
The authors are thankful to the Director of the Indian Veterinary Research Institute, Izatnagar 243 122, India for providing the necessary facilities for the present work.
- Frezza D, Giambra V, Chegdani F et al (2008) Standard and light-cycler PCR methods for animal DNA species detection in animal feedstuffs. IFSET 9:18–23Google Scholar
- Mane BG, Tanwar VK, Girish PS, Dixit VP (2006) Identification of species origin of meat by RAPD-PCR technique. J Vet Public Health 4:87–90Google Scholar
- Mane BG, Mendiratta SK, Tiwari AK, Bhilegaokar KN, Ravindra PV, Raut AA (2007) Precise and rapid identification of ovine-caprine origin of meat by specific PCR assay. J Vet Public Health 5:107–111Google Scholar
- Mane BG, Mendiratta SK, Tiwari AK, Sharma BD, Bhilegaokar KN, Anjaneyulu ASR (2011) Detection of pork in admixed meat and meat products by species-specific PCR technique. Indian J Animal Sci (in press)Google Scholar
- Meyer R, Canadrian U, Luethy J (1994) Detection of pork in heated meat products by the polymerase chain reaction. J AOAC Int 77:617–622Google Scholar
- Piknova L, Kuchta T (2002) Detection of the beef component in meat products using polymerase chain reaction. Bull Potravin Vysk 41:107–111Google Scholar
- Unseld M, Beyermann B, Brandt P, Hiesel R (1995) Identification of the species origin of highly processed meat products by mitochondrial DNA sequences. PCR Meth Appl 4:241–243Google Scholar