Direct Determination of β-Hydroxy-β-Methylbutyrate (HMB) in Liquid Nutritional Products
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A high performance liquid chromatography/ultraviolet method developed for the simple and direct determination of β-hydroxy-β-methylbutyrate (HMB) in liquid nutritional products and dietary supplements is described. Method suitability was defined by experimental assessments of linearity (r 2 > 0.9999), precision (day-to-day RSD <1.5%), accuracy (spike recoveries = 98.1–102.4%, n = 28), selectivity (peak purity average = 99.8 ± 0.8%, n = 10; absence of interference verified by placebo analysis), and quantitation limit (90 mg/kg or 0.8 mM). The method provides for a simple, accurate, and precise quantification of HMB, when present at millimolar concentrations in liquid nutritional products and dietary supplements.
Keywordsβ-Hydroxy-β-methylbutyrate HPLC determination Method validation Liquid nutritional product Hydroxy acid UV absorptivity
The authors gratefully acknowledge the valuable contributions of Keith B. Wheeler and William J. Winter.
- Baier S, Johannsen D, Abumrad N, Rathmacher JA, Nissen S, Flakoll P (2009) Year-long changes in protein metabolism in elderly men and women supplemented with a nutrition cocktail of β-hydroxy-β-methylbutyrate (HMB), l-arginine, and l-lysine. JPEN 33:71–82Google Scholar
- Berk L, James J, Schwartz A, Hug E, Mahadevan A, Samuels M, Kachnic L (2008) A randomized, double-blind, placebo-controlled trial of a β-hydroxyl β-methyl butyrate, glutamine, and arginine mixture for the treatment of cancer cachexia (RTOG 0122). Support Care Cancer 16:1179–1188CrossRefGoogle Scholar
- Kuipers BJH, Gruppen H (2007) Prediction of molar extinction coefficients of proteins and peptides using UV absorption of the constituent amino acids at 214 nm to enable quantitative reverse phase high-performance liquid chromatography-mass spectrometry analysis. J Agric Food Chem 55:5445–5451CrossRefGoogle Scholar
- Sealey WM, Teague AM, Stratton SL, Mock DM (2004) Smoking accelerates biotin catabolism in women. Am J Clin Nutr 80:932–935Google Scholar
- Watanabe T, Oguchi KI, Ebara S, Fukui T (2005) Measurement of 3-hydroxyisovaleric acid in urine of biotin-deficient infants and mice by HPLC. J Nutr 135:615–618Google Scholar