Food Analytical Methods

, Volume 4, Issue 3, pp 341–346 | Cite as

Direct Determination of β-Hydroxy-β-Methylbutyrate (HMB) in Liquid Nutritional Products

  • Jeffrey H. Baxter
  • Rosalyn R. Phillips
  • Lobat Dowlati
  • Karen C. Goehring
  • Paul W. Johns


A high performance liquid chromatography/ultraviolet method developed for the simple and direct determination of β-hydroxy-β-methylbutyrate (HMB) in liquid nutritional products and dietary supplements is described. Method suitability was defined by experimental assessments of linearity (r 2 > 0.9999), precision (day-to-day RSD <1.5%), accuracy (spike recoveries = 98.1–102.4%, n = 28), selectivity (peak purity average = 99.8 ± 0.8%, n = 10; absence of interference verified by placebo analysis), and quantitation limit (90 mg/kg or 0.8 mM). The method provides for a simple, accurate, and precise quantification of HMB, when present at millimolar concentrations in liquid nutritional products and dietary supplements.


β-Hydroxy-β-methylbutyrate HPLC determination Method validation Liquid nutritional product Hydroxy acid UV absorptivity 



The authors gratefully acknowledge the valuable contributions of Keith B. Wheeler and William J. Winter.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Jeffrey H. Baxter
    • 1
  • Rosalyn R. Phillips
    • 1
  • Lobat Dowlati
    • 1
  • Karen C. Goehring
    • 1
  • Paul W. Johns
    • 1
  1. 1.Abbott Laboratories, Abbott Nutrition DivisionColumbusUSA

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