Visual and Rapid Detection of Two Genetically Modified Soybean Events Using Loop-mediated Isothermal Amplification Method
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To develop effective alternatives for detecting genetically modified organisms (GMOs), we reported one optimized visual loop-mediated isothermal amplification (LAMP) method for the detection of exogenous DNA targets from two GM soybean events in this study. This isothermal amplification can be performed within 40 min without polymerase chain reaction (PCR) equipment and the derived LAMP products can be directly observed by naked eye employing SybrGreen I dye instead of conventional gel electrophoresis analysis. The limits of detection of these established visual LAMP assays were about 4 copies of haploid soybean genomic DNA, and which were much higher than those of reported conventional PCR assays. Furthermore, the high specificity of LAMP assays was determined. All the results demonstrated that the developed visual LAMP assays are convenient, cost-efficient, and rapid for on spot detection of GMOs.
KeywordsLoop-mediated Isothermal Amplification Visual Detection Genetically Modified Soybean
This work was supported by the National Key Basic Research Program (2007CB109201, 2007FY230100), the National Transgenic Plant Special Fund (2008ZX08012-002, 003,005, and 2009ZX08012-002B), the National Natural Science Foundation of China (30725022, 30700499), the national high-tech project “863” (2006AA10Z443), and Shanghai Chenguang project (2008CG16).
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