Food Analytical Methods

, 2:264 | Cite as

Solid Phase Extraction Combined Direct Spectrophotometric Determination of Brilliant Blue in Food Using β-Cyclodextrin Polymer



A new and sensitive solid phase extraction (SPE) combined direct spectrophotometry for determination of brilliant blue (BB) in food has been developed, in which β-cyclodextrin polymer (β-CDP) is used as a SPE adsorbent to extract BB from aqueous solutions to form a solid supramolecular complex with a 1:3 molar ratio of BB to β-CDP, and BB in β-CDP was directly determined by a solid phase spectrophotometry. SPE conditions were pH 7.0, temperature 20 °C, ionic strength 0.1 mol/l, and shaking time 40 min. The calibration curve is linearity in the concentration range of 0.05–12.0 μg/ml. The proposed method was applied to the determination of BB in food samples at 627 nm maximum absorption wavelength with satisfactory results.


Solid Phase Extraction Solid Phase Spectrophotometry β-Cyclodextrin Polymer (β-CDP) Brilliant Blue Food Samples 



The authors hope to acknowledge the Key Scientific and Technologic Support Foundation of Tianjin and the Natural Science Foundation of China for financial supports.


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.College of Biotechnology and Food Science, Tianjin Key Laboratory of Food BiotechnologyTianjin University of CommerceTianjinPeople’s Republic of China

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