Food Analytical Methods

, Volume 2, Issue 1, pp 14–22 | Cite as

Detection of Salmonella Enteriditis from Egg Components Using Different Immunomagnetic Beads and Time-resolved Fluorescence

  • Shu-I Tu
  • Sue A. Reed
  • Andrew G. Gehring
  • Yiping He
Article
First Online:
Received:
Accepted:

Abstract

The types of chemical linkage used to bind antibodies to magnetic beads to form immunomagnetic beads (IMB) were compared in the capture and detection of Salmonella Enteriditis from egg white, egg yolk, and whole egg. Egg components were inoculated with outbreak strains of S. Enteriditis. After incubation under different conditions, IMBs derived from linking antibodies to core magnetic beads via biotin–streptavidin interactions, Schiff-base bonds and unspecified proprietary chemistry were used to capture S. Enteriditis. Europium-labeled anti-Salmonella antibodies completed the sandwich, and time-resolved fluorescence served as the means of detection. For the Salmonella isolated in stationary phase and cultured from universal pre-enrichment broth (UPB), the detection signal intensity was affected by the chemistry utilized to link the antibodies to IMB, with results varying among the three test strains. When S. Enteriditis was cultured in egg yolk alone, plating data were similar to those of the growth of S. Enteriditis in UPB. Egg white by itself did not support the growth of S. Enteriditis. The addition of UPB to egg white restored the growth of Salmonella and yielded stronger detection signals than from cultures obtained from UPB with egg yolk. The detection signals obtained from the immunoassay were less intense for cultures grown in egg yolk + UPB than from cultures grown in UPB alone. The lower detection signals elicited by all IMBs suggest the availability of the antigenic groups recognized by the antibodies on IMBs was reduced in the presence of egg yolk.

Keywords

Salmonella Enteriditis Immunomagnetic Beads Time-resolved Fluorescence Food Safety Egg Components 
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References

  1. Baron F, Gautier M, Brule G (1997) J Food Prot 60:1318Google Scholar
  2. Baron F, Briandet R, Lesne J, Humbert F, Ablain W, Gautier M (2004) J Food Prot 67:2269Google Scholar
  3. Braun P, Fehlhaber K (1995) Int J Food Microbiol 25:95CrossRefGoogle Scholar
  4. Che Y, Li Y, Slavik M (2001) Biosens Bioelectron 16:791CrossRefGoogle Scholar
  5. Chen H, Anantheswaran RC, Knabel SJ (2001) J Food Prot 64:1279Google Scholar
  6. Centers for Disease Control (2003) Morbid Mortal Weekly Rep 51:1149Google Scholar
  7. Cudjoe KS, Krona R, Grøn B, Olsen E (1994) Int J Food Microbiol 23:149CrossRefGoogle Scholar
  8. Cudjoe KS, Hagtvedt T, Dainty R (1995) Int J Food Microbiol 27:11CrossRefGoogle Scholar
  9. Duncanson P, Wareing DRA, Jones O (2003) Lett Appl Microbiol 37:144CrossRefGoogle Scholar
  11. Fu Z, Rogelj S, Kieft TL (2005) Int J Food Microbiol 99:47CrossRefGoogle Scholar
  12. Gast RK, Holt PS (1998) J Food Prot 61:107Google Scholar
  13. Gast RK, Holt PS (2003) J Food Prot 66:656Google Scholar
  14. Gehring AG, Irwin PL, Reed SA et al (2004) J Immunol Methods 293:97CrossRefGoogle Scholar
  15. Hamal KR, Burgess SC, Pevzner IY, Erf GF (2006) Poultry Sci 85:1364Google Scholar
  16. Hammack TS, Sherrod PS, Bruce VR, June GA, Satchell FB, Andrews WH (1993) Poultry Sci 72:373Google Scholar
  17. Hara-Kudo Y, Kumagai S, Masuda T et al (2001) Int J Food Microbiol 64:395CrossRefGoogle Scholar
  18. Molday RS, Yen SPS, Rembaum A (1977) Nature 268:437CrossRefGoogle Scholar
  19. Price CA (1982) Centrifugation in density gradients. Academic, New YorkGoogle Scholar
  20. Rijpens N, Herman L, Vereecken F, Jannes G, De Smedt J, De Zutter L (1999) Int J Food Microbiol 46:37CrossRefGoogle Scholar
  21. Stephenson P, Satchell FB, Allen G, Andrews WH (1991) J Assoc Off Anal Chem 74:821Google Scholar
  22. Tu S-I, Golden M, Fett WF, Gehring A, Irwin P (2003a) J Food Safety 23:75CrossRefGoogle Scholar
  23. Tu S, Uknalis J, Gore M, Irwin P, Feder I (2003b) J Rapid Methods Autom Microbiol 11:35Google Scholar
  24. Tu S-I, Uknalis J, Yamashoji S, Gehring A, Irwin P (2005) J Rapid Methods Autom Microbiol 13:57CrossRefGoogle Scholar
  25. Tu S-I, Gehring A, Paoli GC (2007) J Rapid Methods Autom Microbiol 15:107CrossRefGoogle Scholar
  26. U.S. Department of Agriculture (2004) Food safety and inspection service, microbiology laboratory guidebook online. http://www.fsis.usda.gov/PDF/MLG_4_03.pdf
  27. U.S. Food and Drug Administration (2006) Bacteriological analytical manual online. http://www.cfsan.fda.gov/~ebam/bam-5.html
  28. Valenti P, Antonini G, Rossi Fanelli MR, Orsi N, Antonini E (1982) Antimicrob Agents Chemother 21:840Google Scholar
  29. Widjojoatmodjo MN, Fluit AC, Torensma R, Verhoef J (1993) J Immunol Methods 165:11CrossRefGoogle Scholar
  30. Yu H (1998) Anal Chim Acta 376:77CrossRefGoogle Scholar
  31. Yu LSL, Uknalis J, Tu S-I (2001) J Immunol Methods 256:11CrossRefGoogle Scholar
  32. Yu LSL, Reed SA, Golden MH (2002) J Microbiol Methods 49:63CrossRefGoogle Scholar
  33. Yu LSL, Reed SA, Tarkkinen P, Tu S-I (2003) J Rapid Methods Autom Microbiol 11:133CrossRefGoogle Scholar
  34. Zarzecka A, Bartoszcze M (2006) J Vet Med 53:393CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Shu-I Tu
    • 1
  • Sue A. Reed
    • 1
  • Andrew G. Gehring
    • 1
  • Yiping He
    • 1
  1. 1.Eastern Regional Research Center, Agricultural Research ServiceUnited States Department of AgricultureWyndmoorUSA

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