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BioEnergy Research

, Volume 12, Issue 1, pp 103–111 | Cite as

A Steam-Explosion-Based Hydrolysis and Acidification Technology for Cornstalk Bioconversion

  • Lan Wang
  • Yang Liu
  • Hongzhang ChenEmail author
Article
  • 193 Downloads

Abstract

Acidification is a potential solution to lignocellulose conversion. In this work, steam explosion was proposed to pretreat cornstalk and improve acidification bioconversion efficiency. Results showed that steam explosion improved the conversion ratio by 17% compared with the control group. The optimum temperature of the hydrolysis and acidification system was 50 °C and the microbial community showed higher hydrolysis and acidification efficiency at pH 8.0–9.0, which were 66.0% (pH 8.0) and 68.0% (pH 9.0), respectively. An organic nitrogen source was more preferred by the microbial community than an inorganic nitrogen source. The highest conversion ratio (67.0%) was observed when the yeast extract dose was 1.0 g/L. Steam-exploded cornstalk was degraded by the microbial community, and organic acids in hydrolysis and acidification liquid was effectively reused by R. eutropha H16 (The utilization ratios of acetic acid, propionic acid and butyric acid were 53.38, 12.27 and 20.95%, respectively.). Based on this study, steam-explosion-based conversion technology is proposed and has great potential in future application of lignocellulose conversion.

Keywords

Steam explosion Hydrolysis and acidification Process regulation Cornstalk 

Notes

Acknowledgements

The authors gratefully acknowledge the financial support from the Transformational Technologies for Clean Energy and Demonstration (Strategic Priority Research Program of the Chinese Academy of Sciences, grant no. XDA 21060300), the Special Project of Cultivation and Development of Innovation Base of Beijing (grant no. Z171100002217003), and the Equipment Project of the State Key Laboratory of Biochemical Engineering (grant no. Y826051101) in this work.

References

  1. 1.
    Chen HZ (2014) Biotechnology of lignocellulose. Springer, Netherlands, pp 403–510Google Scholar
  2. 2.
    Bosch SVD, Schutyser W, Vanholme R, Driessen T, Koelewijn SF, Renders T, Meester BD, Huijgen WJJ, Dehaen W, Courtin MC (2015) Reductive lignocellulose fractionation into soluble lignin-derived phenolic monomers and dimers and processable carbohydrate pulps. Energy Environ Sci 8(6):1748–1763Google Scholar
  3. 3.
    Kumar G, Bakonyi P, Periyasamy S, Kim SH, Nemestóthy N, Bélafi-Bakó K (2015) Lignocellulose biohydrogen: practical challenges and recent progress. Renew Sust Energ Rev 44:728–737Google Scholar
  4. 4.
    Jin MJ, Gunawan C, Uppugundla N, Balan V, Dale BE (2012) A novel integrated biological process for cellulosic ethanol production featuring high ethanol productivity, enzyme recycling and yeast cells reuse. Energy Environ Sci 5(5):7168–7175Google Scholar
  5. 5.
    Zheng Y, Pan Z, Zhang R, Wang D (2009) Enzymatic saccharification of dilute acid pretreated saline crops for fermentable sugar production. Appl Energy 86(11):2459–2465Google Scholar
  6. 6.
    Chen WH, Benli P, Yu CT, Wensong H (2011) Pretreatment efficiency and structural characterization of rice straw by an integrated process of dilute-acid and steam explosion for bioethanol production. Bioresour Technol 102(3):2916–2924PubMedGoogle Scholar
  7. 7.
    Himmel ME, Ding SY, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315(5813):804–807Google Scholar
  8. 8.
    Chen HZ, Liu ZH (2014) Multilevel composition fractionation process for high-value utilization of wheat straw cellulose. Biotechnol Biofuels 7(1):1–12Google Scholar
  9. 9.
    Alvira P, Tomás-Pejó E, Ballesteros M, Negro MJ (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour Technol 101(13):4851–4861Google Scholar
  10. 10.
    Chen HZ, Liu LY (2007) Unpolluted fractionation of wheat straw by steam explosion and ethanol extraction. Bioresour Technol 98(3):666–676Google Scholar
  11. 11.
    Liu ZH, Qin L, Jin MJ, Pang F, Li BZ, Kang Y, Dale BE, Yuan YJ (2013) Evaluation of storage methods for the conversion of corn Stover biomass to sugars based on steam explosion pretreatment. Bioresour Technol 132(2):5–15PubMedGoogle Scholar
  12. 12.
    Zhang LH, Li D, Wang LJ, Wang TP, Zhang L, Chen XD, Mao ZH (2008) Effect of steam explosion on biodegradation of lignin in wheat straw. Bioresour Technol 99(17):8512–8515PubMedGoogle Scholar
  13. 13.
    Leu SY, Zhu JY (2013) Substrate-related factors affecting enzymatic saccharification of lignocelluloses: our recent understanding. BioEnergy Res 6(2):405–415Google Scholar
  14. 14.
    Marmann A, Aly AH, Lin W, Wang B, Proksch P (2014) Co-cultivation--a powerful emerging tool for enhancing the chemical diversity of microorganisms. Mar Drugs 12(2):1043PubMedPubMedCentralGoogle Scholar
  15. 15.
    Horiuchi JI, Shimizu T, Tada K, Kanno T, Kobayashi M (2002) Selective production of organic acids in anaerobic acid reactor by pH control. Bioresour Technol 82(3):209PubMedGoogle Scholar
  16. 16.
    Zhang X, Qiu WH, Chen HZ (2012) Enhancing the hydrolysis and acidification of steam-exploded cornstalks by intermittent pH adjustment with an enriched microbial community. Bioresour Technol 123(123):30PubMedGoogle Scholar
  17. 17.
    Fernándezdacosta C, Posada JA, Kleerebezem R, Cuellar MC, Ramirez A (2015) Microbial community-based polyhydroxyalkanoates (PHAs) production from wastewater: techno-economic analysis and ex-ante environmental assessment. Bioresour Technol 185:368–377Google Scholar
  18. 18.
    Scarlata C, Sluiter J, Templeton D, Crocker D (2011) Determination of structural carbohydrates and lignin in biomass. National Renewable Energy Laboratory-NREL/TP-510-42618 Laboratory Analytical Procedure (LAP) Golden, COGoogle Scholar
  19. 19.
    Mood SH, Golfeshan AH, Tabatabaei M, Jouzani GS, Najafi GH, Gholami M, Ardjmand M (2013) Lignocellulosic biomass to bioethanol, a comprehensive review with a focus on pretreatment. Renew Sust Energ Rev 27(6):77–93Google Scholar
  20. 20.
    Chen HZ (2013) Technology of steam explosion and biorefining. Springer, Netherlands, pp 35–37Google Scholar
  21. 21.
    Zhao JY, Chen HZ (2013) Correlation of porous structure, mass transfer and enzymatic hydrolysis of steam exploded corn Stover. Chem Eng Sci 104(50):1036–1044Google Scholar
  22. 22.
    Lee S, Yu J (1997) Production of biodegradable thermoplastics from municipal sludge by a two-stage bioprocess. Resour Conserv Recycl 19(3):151–164Google Scholar
  23. 23.
    Temudo MF, Kleerebezem R, Loosdrecht MV (2007) Influence of the pH on (open) mixed culture fermentation of glucose: a chemostat study. Biotechnol Bioeng 98(1):69PubMedGoogle Scholar
  24. 24.
    Lin CY, Lay CH (2004) Carbon/nitrogen-ratio effect on fermentative hydrogen production by mixed microflora. Int J Hydrog Energy 29(1):41–45Google Scholar
  25. 25.
    Wang Y, Wang H, Feng XQ, Wang XF, Huang JX (2010) Biohydrogen production from cornstalk wastes by anaerobic fermentation with activated sludge. Int J Hydrog Energy 35(7):3092–3099Google Scholar
  26. 26.
    Steinbüchel A, Schlegel HG (1991) Physiology and molecular genetics of poly(beta-hydroxy-alkanoic acid) synthesis in Alcaligenes eutrophus. Mol Microbiol 5(3):535–542PubMedGoogle Scholar
  27. 27.
    Yang YH, Brigham CJ, Budde CF, Boccazzi P, Willis LB, Hassan MA, Yusof ZAM, Rha C, Sinskey AJ (2010) Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. Appl Microbiol Biotechnol 87(6):2037–2045PubMedGoogle Scholar
  28. 28.
    Fukui T, Chou K, Harada K, Orita I, Nakayama Y, Bamba T, Nakamura S, Fukusaki E (2014) Metabolite profiles of polyhydroxyalkanoate-producing Ralstonia eutropha H16. Metabolomics 10(2):190–202Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Biochemical Engineering, Beijing Key Laboratory of Biomass Refining Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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