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BioEnergy Research

, Volume 11, Issue 3, pp 528–537 | Cite as

Fremyella diplosiphon as a Biodiesel Agent: Identification of Fatty Acid Methyl Esters via Microwave-Assisted Direct In Situ Transesterification

  • Behnam Tabatabai
  • Huan Chen
  • Jie Lu
  • Jamiu Giwa-Otusajo
  • Amy M. McKenna
  • Alok K. Shrivastava
  • Viji SittherEmail author
Article

Abstract

Increasing concerns on environmental and economic issues linked to fossil fuel use has driven great interest in cyanobacteria as third-generation biofuel agents. In this study, the biodiesel potential of a model photosynthetic cyanobacterium, Fremyella diplosiphon, was identified by fatty acid methyl esters (FAME) via direct transesterification. Total lipids in wild type (Fd33) and halotolerant (HSF33-1 and HSF33-2) strains determined by gravimetric analysis yielded 19% cellular dry weight (CDW) for HSF33-1 and 20% CDW for HSF33-2, which were comparable to Fd33 (18% CDW). Gas chromatography-mass spectrometry detected a high ratio of saturated to unsaturated FAMEs (2.48–2.61) in transesterified lipids, with methyl palmitate being the most abundant (C16:0). While theoretical biodiesel properties revealed high cetane number and oxidative stability, high cloud and pour point values indicated that fuel blending could be a viable approach. Significantly high FAME abundance in total transesterified lipids of HSF33-1 (40.2%) and HSF33-2 (69.9%) relative to Fd33 (25.4%) was identified using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry, indicating that robust salt stress response corresponds to higher levels of extractable FAME. Alkanes, a key component in conventional fuels, were present in F. diplosiphon transesterified lipids across all strains confirming that natural synthesis of these hydrocarbons is not inhibited during biodiesel production. While analysis of photosynthetic pigments and phycobiliproteins did not reveal significant differences, FAME abundance varied significantly in wild type and halotolerant strains indicating that photosynthetic pathways are not the sole factors that determine fatty acid production. We characterize the potential of F. diplosiphon for biofuel production with FAME yields in halotolerant strains higher than the wild type with no loss in photosynthetic pigmentation.

Keywords

Alkane Biofuel Lipid Phycobiliprotein 

Notes

Acknowledgments

The study was supported by TEDCO MII [0913-001-3] and the National Institutes of Health [UL1GM118973] awarded to Morgan State University and the National Science Foundation [DMR 11-57490] awarded to the National High Magnetic Field Laboratory and the State of Florida. Assistance provided by the Future Fuels Institute in the Ion Cyclotron Resonance Facility is gratefully acknowledged. The authors thank Dr. Beronda L. Montgomery at Michigan State University for providing the Fd33 strain and Dr. Solomon Tadesse at Morgan State University for the technical help.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiologyMorgan State UniversityBaltimoreUSA
  2. 2.National High Magnetic Field LaboratoryFlorida State UniversityTallahasseeUSA
  3. 3.Future Fuels Institute, Florida State UniversityTallahasseeUSA
  4. 4.Department of BotanyMahatma Gandhi Central UniversityMotihariIndia

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