BioEnergy Research

, Volume 9, Issue 4, pp 1034–1045 | Cite as

Cellulase and Xylanase Production by the Mexican Strain Talaromyces stollii LV186 and Its Application in the Saccharification of Pretreated Corn and Sorghum Stover

  • Montserrat Orencio-Trejo
  • Jessica Torres-Granados
  • Arelis Rangel-Lara
  • Esmeralda Beltrán-Guerrero
  • Santos García-Aguilar
  • Cessna Moss-Acosta
  • Humberto Valenzuela-Soto
  • Susana De la Torre-Zavala
  • Argel Gastelum-Arellanez
  • Alfredo Martinez
  • Axel Tiessen
  • Edith Diaz-Mireles
  • Edmundo Lozoya-Gloria
Article

Abstract

A Mexican strain of Talaromyces stollii LV186 was isolated from decaying pretreated corn stover. The production of cellulase and xylanase enzyme cocktails was evaluated with corn and sorghum stover used as inducers in a mineral medium. The volumetric and specific activities of T. stollii LV186 were compared with the values produced by Trichoderma reesei ATCC 26921 in a time-course experiment. After the submerged culture and a posterior ultrafiltration stage, the enzyme complexes were evaluated over acid-pretreated corn or sorghum stover in baffled flasks under controlled temperature and agitation conditions, and hydrolysis levels of 30 and 39 % of the theoretical maximum were obtained after only 72-h reactions, for each substrate. A side-by-side comparison showed a better ratio of endoglucanase to cellobiohydrolase to β-glucosidase and of xylanase to β-xylosidase enzymes in T. stollii than in T. reesei ATCC 26921. Furthermore, the hydrolysis of pretreated corn and sorghum stover achieved by T. stollii is significantly higher compared with that of a commercial cocktail from T. reesei ATCC 26921 (Celluclast). Therefore, the T. stollii LV186 strain is a good candidate for the hydrolysis of complex lignocellulose substrates. To the authors’ knowledge, this study is the first to describe the cellulolytic and hemicellulolytic activities produced by a T. stollii strain.

Keywords

Cellulases Xylanases Enzymatic hydrolysis Corn and sorghum stover Talaromyces stollii 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Montserrat Orencio-Trejo
    • 1
    • 2
  • Jessica Torres-Granados
    • 1
  • Arelis Rangel-Lara
    • 1
  • Esmeralda Beltrán-Guerrero
    • 1
  • Santos García-Aguilar
    • 1
  • Cessna Moss-Acosta
    • 3
  • Humberto Valenzuela-Soto
    • 1
    • 4
  • Susana De la Torre-Zavala
    • 1
    • 2
  • Argel Gastelum-Arellanez
    • 2
    • 5
  • Alfredo Martinez
    • 3
  • Axel Tiessen
    • 1
  • Edith Diaz-Mireles
    • 1
  • Edmundo Lozoya-Gloria
    • 1
  1. 1.Departamento de Ingeniería GenéticaCINVESTAV IPN Unidad IrapuatoIrapuatoMexico
  2. 2.Instituto de Biotecnología de la Universidad Autónoma de Nuevo LeónSan Nicolás de los GarzaMexico
  3. 3.Departamento de Ingeniería Celular y Biocatálisis, Instituto de BiotecnologíaUniversidad Nacional Autónoma de MéxicoCuernavacaMexico
  4. 4.Departamento de Plásticos en la AgriculturaCentro de Investigación en Química AplicadaSaltilloMexico
  5. 5.Departamento de Biotecnología y BioquímicaCINVESTAV IPN Unidad IrapuatoIrapuatoMexico

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