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BioEnergy Research

, Volume 9, Issue 3, pp 969–979 | Cite as

Eucalyptus Cell Wall Architecture: Clues for Lignocellulosic Biomass Deconstruction

  • Marcela Mendes Salazar
  • Adriana Grandis
  • Sivakumar Pattathil
  • Jorge Lepikson Neto
  • Eduardo Leal Oliveira Camargo
  • Ana Alves
  • José Carlos Rodrigues
  • Fabio Squina
  • João Paulo Franco Cairo
  • Marcos S. Buckeridge
  • Michael G. Hahn
  • Gonçalo Amarante Guimarães Pereira
Article

Abstract

The architecture, composition, and chemical properties of wood cell walls have a direct influence on the process that occurs prior to fermentation in second-generation biofuel production. The understanding of the construction patterns of cell wall types is the key to the new era of second-generation biofuels. Eucalyptus species are great candidates for this purpose since these species are among the fastest growing hardwood trees in the world and they have been improved for biomass production. We applied the glycome profiling and other combined techniques to study xylem cell walls of three economically important species (Eucalyptus globulus, Eucalyptus grandis, and Eucalyptus urophylla). Glycome profiling analyses revealed that species differ in the same key aspects of cell wall polymer linkages, with E. globulus and E. urophylla presenting contrasting phenotypes, and E. grandis with intermediate characteristics. E. urophylla is known for high recalcitrance, that is probably determined by the strong associations between lignin and cell wall polymers, and also lignin content. On the other hand, E. globulus cell wall polymers are loosely linked, so its cell wall can be easily deconstructed. We have shown in this work that the composition of cell walls differs in quantity and quality among the Eucalyptus species and such variations in composition influence the process of lignocellulosic feedstock assessment. However, the greatest influence relies on the amount and type of associations between cell wall polymers. A high yield of cellulose, from any biomass source, directly depends on the cell wall architecture.

Keywords

Bioethanol Cell wall architecture Glycome profiling Eucalyptus Wood 

Abbreviations

1 M

1 Molar

4 M

4 Molar

AIR

Alcohol-insoluble residues

DMSO

Dimethyl sulfoxide

Ara

Arabinose

cP/cH

Pentose/hexose

Fuc-XG

Fucosylated xyloglucan

GalA

Galacturonic acid

Glc

Glucose

Gal

Galactose

HG

Homogalacturonan

H/G

Hydroxyphenyl/guaiacyl

LCC

Lignin-carbohydrate complex

Man

Mannose

S/G

Syringyl/guaiacyl

Non-fuc XG

Non-fucosylated xyloglucan

PC

Post chlorite

RG

Rhamnogalacturonan backbone

RG/AG

Rhamnogalacturonan/arabinogalactan

RG-I

Rhamnogalacturonan I

RG-II

Rhamnogalacturonan II

Rha

Rhamnose

XG

Xyloglucan

Xyl

Xylose

Fuc

Fucose

Notes

Acknowledgments

The authors would like to thank International Paper-Brazil for kindly providing plant materials and Espaço da Escrita/Coordenadoria Geral-UNICAMP for English revision. This work was supported by research funding of FAPESP (process number 2007/54877-0) and grants from International Paper do Brasil (IP/IB/Gene Discovery: 3972).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Marcela Mendes Salazar
    • 1
    • 2
  • Adriana Grandis
    • 3
  • Sivakumar Pattathil
    • 4
  • Jorge Lepikson Neto
    • 1
    • 2
  • Eduardo Leal Oliveira Camargo
    • 1
  • Ana Alves
    • 5
  • José Carlos Rodrigues
    • 5
  • Fabio Squina
    • 6
  • João Paulo Franco Cairo
    • 1
    • 6
  • Marcos S. Buckeridge
    • 3
  • Michael G. Hahn
    • 4
  • Gonçalo Amarante Guimarães Pereira
    • 1
  1. 1.Genomic and Expression Laboratory, Department of Evolution Genetics and Bioagents, Institute of BiologyUniversity of CampinasCampinasBrazil
  2. 2.Senai Innovation Institute for BiomassTrês LagoasBrazil
  3. 3.Department of Botany, Institute of BiosciencesUniversity of São PauloSão PauloBrazil
  4. 4.BioEnergy Science Center, Complex Carbohydrate Research CenterUniversity of GeorgiaAthensUSA
  5. 5.Tropical Research Institute of Portugal (IICT), Forestry and Forest Products GroupLisbonPortugal
  6. 6.Brazilian Bioethanol Science and Technology Laboratory (CTBE)CampinasBrazil

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