BioEnergy Research

, Volume 8, Issue 1, pp 464–470 | Cite as

Effect of Hot-Pressing Temperature on the Subsequent Enzymatic Saccharification and Fermentation Performance of SPORL Pretreated Forest Biomass

  • Jingzhi Zhang
  • Andrea Laguna
  • Craig Clemons
  • Michael P. Wolcott
  • Rolland Gleisner
  • J. Y. ZhuEmail author
  • Xu Zhang


Methods to increase the energy density of biofuel feedstock for shipment are important towards improving supply chain efficiency in upstream processes. Towards this end, densified pretreated lignocellulosic biomass was produced using hot-pressing. The effects of fiber hornification induced by hot-pressing on enzymatic digestibilities of lodgepole pine and poplar NE222 wood chips pretreated by sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) were examined. Pretreated wood chips were pressed at 25, 70, 90, 110, and 177 °C. The cellulose accessibilities of the pressed and unpressed substrates were evaluated using water retention value and direct cellulase adsorption measurements. Hot-pressing below 110 °C produced a degree of hornification (DH) below 0.26 and had limited effect on cellulose accessibility and enzymatic digestibility. Hot-pressing at 177 °C produced a DH of 0.86 that substantially hornified the fibers and resulted near zero saccharification. The saccharification results were consistent with cellulose accessibility data. Ethanol fermentation studies at 18 % solids suggest that a pressing below 110 °C is preferred to reduce its effect on biofuel yield.


Biomass commoditization Supply chain logistics Densification Water retention value Cellulase adsorption Enzymatic hydrolysis 



We would like to acknowledge the financial support from the Agriculture and Food Research Initiative Competitive grant (no. 2011-68005-30416) and USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA) and the Chinese Scholarship Council (CSC). These two programs made the visiting appointment of Jinzhi Zhang at the USDA Forest Products Lab (FPL) possible. The USDA-NIFA funding also supported Andrea Laguna’s summer internship at FPL through the Washington State University Summer Undergraduate Research Program. We also would like to acknowledge Phil Walsh and Fred Matt of FPL for pressing the chips and carbohydrate analysis, respectively.


  1. 1.
    Miao Z, Shastri Y, Grift TE, Hansen AC, Ting KC (2012) Lignocellulosic biomass feedstock transportation alternatives, logistics, equipment configurations, and modeling. Biofuels Bioprod Biorefin 6(3):351–362CrossRefGoogle Scholar
  2. 2.
    Thompson DN, Campbell T, Bals B, Runge T, Teymouri F, Ovard LP (2013) Chemical preconversion: application of low-severity pretreatment chemistries for commoditization of lignocellulosic feedstock. Biofuels 4(3):323–340CrossRefGoogle Scholar
  3. 3.
    Stephen JD, Mabee WE, Saddler JN (2010) Biomass logistics as a determinant of second-generation biofuel facility scale, location, and technology selection. Biofpr 4:503–518Google Scholar
  4. 4.
    Hess JR, Kenney KL, Ovard LP, Searcy EM, Wright CT: Commodity-scale production of an infrastructure-compatible bulk solid from herbaceous lignocellulosic biomass. Idaho National Laboratory, Contract No: INL/EXT-09-17527 2009Google Scholar
  5. 5.
    Tumuluru JS, Wright CT, Hess JR, Kenney KL (2011) A review of biomass densification systems to develop uniform feedstock commodities for bioenergy application. Biofuels Bioprod Biorefin 5(6):683–707CrossRefGoogle Scholar
  6. 6.
    Ray AE, Hoover AN, Nagle N, Chen X, Gresham GL (2013) Effect of pelleting on the recalcitrance and bioconversion of dilute-acid pretreated corn stover under low- and high-solids conditions. Biofuels 4(3):271–284CrossRefGoogle Scholar
  7. 7.
    Kumar L, Tooyserkani Z, Sokhansanj S, Saddler JN (2012) Does densification influence the steam pretreatment and enzymatic hydrolysis of softwoods to sugars? Bioresour Technol 121:190–198CrossRefPubMedGoogle Scholar
  8. 8.
    Nahar N, Pryor SW (2014) Reduced pretreatment severity and enzyme loading enabled through switchgrass pelleting. Biomass Bioenergy 67:46–52CrossRefGoogle Scholar
  9. 9.
    Luo X, Zhu JY (2011) Effects of drying-induced fiber hornification on enzymatic saccharification of lignocelluloses. Enzym Microb Technol 48(1):92–99CrossRefGoogle Scholar
  10. 10.
    Luo X, Zhu JY, Gleisner R, Zhan HY (2011) Effect of wet pressing-induced fiber hornification on enzymatic saccharification of lignocelluloses. Cellulose 18:1339–1344CrossRefGoogle Scholar
  11. 11.
    Stone JE, Scallan AM (1965) Influence of drying on the pore structures of the cell wall. In: 3rd Fundamental Research Symposium; Cambridge, UK. Pulp and Paper Fundamental Research Society: 145–174Google Scholar
  12. 12.
    Carolan JE, Joshi SV, Dale BE (2007) Technical and financial feasibility analysis of distributed bioprocessing using regional biomass pre-processing centers. Journal of Agricultural and Food Industrial Organization 5(2)Google Scholar
  13. 13.
    Bals BD, Gunawan C, Moore J, Teymouri F, Dale BE (2014) Enzymatic hydrolysis of pelletized AFEX™-treated corn stover at high solid loadings. Biotechnol Bioeng 111(2):264–271CrossRefPubMedGoogle Scholar
  14. 14.
    Zhu JY, Pan XJ, Wang GS, Gleisner R (2009) Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine. Bioresour Technol 100(8):2411–2418CrossRefPubMedGoogle Scholar
  15. 15.
    Wang ZJ, Zhu JY, Gleisner R, Chen KF (2012) Ethanol production form poplar wood the rough enzymatic saccharification and fermentation by dilute acid and SPORL pretreatments. Fuel 95:606–614CrossRefGoogle Scholar
  16. 16.
    Zhou H, Zhu JY, Luo X, Leu S-Y, Wu X, Gleisner R, Dien BS, Hector RE, Yang D, Qiu X et al (2013) Bioconversion of beetle-killed lodgepole pine using SPORL: Process scale-up design, lignin coproduct, and high solids fermentation without detoxification. Ind Eng Chem Res 52(45):16057–16065CrossRefGoogle Scholar
  17. 17.
    Wood TM, Bhat M: Methods for measuring cellulase activities. In: In: Colowick SP, Kaplan NO, editors Methods in Enzymology, Vol 160, Biomass (Part A, Cellulose and Hemicellulose) Vol editors:Wood WA, Kellogg ST New York: Academic Press, Inc, p 87–112. 1988: 87–112Google Scholar
  18. 18.
    SCAN: SCAN-C 62:00 Water retention value of chemical pulps. Nordic Standardization Program 2000Google Scholar
  19. 19.
    Lan TQ, Lou H, Zhu JY (2013) Enzymatic saccharification of lignocelluloses should be conducted at elevated pH 5.2–6.2. Bioenerg Res 6(2):476–485CrossRefGoogle Scholar
  20. 20.
    Lou H, Zhu JY, Lan TQ, Lai H, Qiu X (2013) pH-induced lignin surface modification to reduce nonspecific cellulase binding and enhance enzymatic saccharification of lignocelluloses. ChemSusChem 6(5):919–927CrossRefPubMedGoogle Scholar
  21. 21.
    Zhou H, Leu S-Y, Wu X, Zhu JY, Gleisner R, Yang D, Qiu X, Horn E (2014) Comparisons of high titer ethanol production and lignosulfonate properties by SPORL pretreatment of lodgepole pine at two temperatures. RSC Adv 4:27033–27038Google Scholar
  22. 22.
    Segerström M, Larsson SH (2014) Clarifying sub-processes in continuous ring die pelletizing through die temperature control. Fuel Process Technol 123:122–126CrossRefGoogle Scholar
  23. 23.
    Wang QQ, He Z, Zhu Z, Zhang Y-HP, Ni Y, Luo XL, Zhu JY (2012) Evaluations of cellulose accessibilities of lignocelluloses by solute exclusion and protein adsorption techniques. Biotechnol Bioeng 109(2):381–389CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York (outside the USA) 2014

Authors and Affiliations

  • Jingzhi Zhang
    • 1
    • 2
  • Andrea Laguna
    • 2
    • 3
  • Craig Clemons
    • 2
  • Michael P. Wolcott
    • 4
  • Rolland Gleisner
    • 2
  • J. Y. Zhu
    • 2
  • Xu Zhang
    • 1
  1. 1.School of Life Science and TechnologyBeijing University of Chemical TechnologyBeijingChina
  2. 2.Forest Products LabUSDA Forest ServiceMadisonUSA
  3. 3.University of Wisconsin-MadisonMadisonUSA
  4. 4.Department of Civil and Environmental EngineeringWashington State UniversityPullmanUSA

Personalised recommendations