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BioEnergy Research

, Volume 6, Issue 3, pp 984–990 | Cite as

Marker-Aided Selection of Polyploid Poplars

  • Fanming Kong
  • Jingjing Liu
  • Yingnan Chen
  • Zhibing Wan
  • Tongming YinEmail author
Article

Abstract

Polyploid breeding is an important means for creating elite varieties for the development of poplar plantations. However, polyploid poplars are rare in natural stands. In this study, we established an analytical toolkit to perform marker-aided selection of polyploid poplars. This toolkit contains 12 SSR primer pairs with sites located in the exonic DNA regions and resulting amplified microsatellites in the intronic/intergenic regions. Highly conserved primer pairs were selected by testing in eight species from four poplar sections. The amplified loci’s variability was examined using trees from a germplasm collection of Populus deltoides. Subsequently, copy numbers amplified by the highly variable primers were experimentally determined using progeny of a full-sib diploid pedigree. Based on the above tests, a subset of primers were finally selected and used for marker-aided selection of polyploid poplars from a set of natural Populus tomentosa stands. The reliability of the established analytical toolkit was further verified using a flow cytometer. We established a fast and reliable technique to screen polyploid poplars from natural stands.

Keywords

Marker-aided selection Polyploidy Microsatellite Poplar 

Notes

Acknowledgments

Funding for this work was provided by the Key Forestry Public Welfare Project (201304102), the 973 project (2012CB114505), and the Natural Science Foundation of China (31125008). It was also funded by the Doctorate Fellowship Foundation (CXZZ11_0508) and the PCSIRT program of Jiangsu province and the Chinese Educational Department.

Supplementary material

12155_2013_9331_MOESM1_ESM.doc (114 kb)
Supplemental Table 1 Alleles generated by 12 SSR primer pairs in 16 P. tomentosa trees. Note: Digital numbers in this table refer to the allele’s size. Loci with only one allele are homozygous, and those with more than one allele are heterozygous. Shadow labeled cells indicate samples that have three alleles at the corresponding loci. (DOC 114 kb)
12155_2013_9331_MOESM2_ESM.xls (90 kb)
Supplemental Table 2 Marker segregation in a F1 pedigree (P. deltoides × P. cathayana). Note: Digital numbers in column of “peaks” refer to the size of amplicons by different primers, and “Fail” means unsuccessful amplification. Fully informative markers are those primers amplified heterozygous locus in both parents. The primers in gray shadow generate fully informative markers but peaks are not neat. The primers in yellow shadow generate fully informative markers with neat peaks and they are used in marker-aided selection for polyploidy poplars. These primers were subsequently renamed as Ploidp-01 toPloidp-10. (XLS 89 kb)

References

  1. 1.
    Meyers LA, Levin DA (2006) On the abundance of polyploids in flowering plants. Evolution 60:1198–1206PubMedGoogle Scholar
  2. 2.
    Otto SP (2007) The evolutionary consequences of polyploidy. Cell 131:452–462PubMedCrossRefGoogle Scholar
  3. 3.
    Cavalier-Smith T (1978) Nuclear volume control by nucleoskeletal DNA, selection for cell volume and cell growth rate, and the solution of the DNA C-value paradox. J Cell Sci 34:247–278PubMedGoogle Scholar
  4. 4.
    Gregory TR (2001) Coincidence, coevolution, or causation? DNA content, cell size, and the C-value enigma. Biol Rev Camb Philos Soc 76:65–101PubMedCrossRefGoogle Scholar
  5. 5.
    Gregory TR, Mable BK (2005) Polyploidy in animals. In: Gregory TR (ed) The evolution of the genome. San Diego, Elsevier, pp 427–517CrossRefGoogle Scholar
  6. 6.
    Einspahr DW (1984) Production and utilization of triploid hybrid Aspen. Iowa State J Res 58:401–409Google Scholar
  7. 7.
    Li Y, Feng DL (2005) Advances in research into polyploidy breeding of woody plants. Chin Bullof Botany 22:375–382Google Scholar
  8. 8.
    Eckenwalder JE (1996) Systematics and evolution of Populus. In: Stettler RF, Bradshaw HD Jr, Heilman PE, Hinckley TM (eds) Biology of Populus and its implications for management and conservation. NRC Research, Ottawa, pp 7–32Google Scholar
  9. 9.
    Smith EC (1943) A study of cytology and speciation in the genus Populus L. J Arnold Arbor 24:275–305Google Scholar
  10. 10.
    Bradshaw HD III, Stettler RF (1993) Molecular genetics of growth and development in Populus. I. Triploidy in hybrid poplars. Theor Appl Genet 86:301–307Google Scholar
  11. 11.
    Nilsson-Ehle H (1936) Note regarding the gigas form of Populus tremula found in nature. Hereditas 21:372–382Google Scholar
  12. 12.
    Johnsson H (1942) Cytological studies of triploid progenies of Populus tremula. Hereditas 28:306–312CrossRefGoogle Scholar
  13. 13.
    Johnsson H (1945) Chromosome numbers of the progeny from the cross triploid × tetraploid Populus tremula. Hereditas 31:500–501PubMedGoogle Scholar
  14. 14.
    Johnsson H (1945) The triploid progeny of the cross diploid × tetraploid Populus tremula. Hereditas 31:411–440PubMedCrossRefGoogle Scholar
  15. 15.
    Johnsson H (1953) Development of triploid and diploid Populus tremula during the juvenile period. Z Forstgenet 2:73–77Google Scholar
  16. 16.
    Einspahr DW, Wyckoff GW (1975) Aspen hybrids—a future source of Lake states fiber. Pulp and Paper 49:118–119Google Scholar
  17. 17.
    Weisgerber H, Rau HM, Gartner EJ et al (1980) 25 years of forest tree breeding in Hessen. Allg Forestz 26:665–712Google Scholar
  18. 18.
    Zhu ZD, Kang XY, Zhang ZY (1998) Studies on selection of natural triploids of Populus tomentosa. Sci Sil Sin 34:22–30Google Scholar
  19. 19.
    Zhao JF, Wang HB, Feng DJ, Wang XA (2001) Structure characteristics and variability of the triploid of Populus tomentosa. Shanxi Forest Sci & Tech 4(1–3):22Google Scholar
  20. 20.
    Xu AQ, Fan YM, Zhang ZY, Xie YM (2005) The growing features and chemical composition variation of triploid Populus tomentosa (B304). China Pulp& Paper Industry 26:63–65Google Scholar
  21. 21.
    Kang XY (2010) Some understandings on polyploid breeding of poplars. J Beijing Forestry University 32:149–153Google Scholar
  22. 22.
    Yin TM, DiFazio SP, Gunter LE et al (2008) Genome structure and emerging evidence of an incipient sex chromosome in Populus. Genome Res 18:422–430PubMedCrossRefGoogle Scholar
  23. 23.
    Yin TM, Zhang XY, Gunter L et al (2009) Microsatellite primers resource developed from the mapped sequence scaffolds of Nisqually-1 genome. New Phytol 181:498–503PubMedCrossRefGoogle Scholar
  24. 24.
    Tuskan GA, Gunter LE, Yang ZM, Yin TM, Sewell MM, DiFazio SP (2004) Characterization of microsatellites revealed by genomic sequencing of Populus trichocarpa. Can J For Res 34:5–93CrossRefGoogle Scholar
  25. 25.
    Keim P, Beavis W, Schupp J, Freestone R (1992) Evaluation of soybean RFLP marker diversity in adapted germplasm. Theor Appl Genet 85:205–212Google Scholar
  26. 26.
    Yin TM, Difazio SP, Gunter LE, Riemenschneider D, Tuskan GA (2004) Large-scale heterospecific segregation distortion in Populus revealed by a dense genetic map. Theo Appl Genet 109:451–463CrossRefGoogle Scholar
  27. 27.
    Otto F (1990) DAPI staining of fixed cells for high-resolution low cytometry of nuclear DNA. In: Crissman HA, Darzynkiewicz Z (eds) Methods in cell biology, vol 33. Academic, New York, pp 105–110Google Scholar
  28. 28.
    Stettler RF, Bradshaw HD Jr, Heilman PE, Hinckley TM (1996) Biology of Populus and its implications for management and conservation. Part I. CRC, OttawaGoogle Scholar
  29. 29.
    Klopfenstein NB, Chun YW, Kim MS, Ahuja MR (1997) Micropropagation, genetic engineering, and molecular biology of Populus. General technical report RM-GTR-297. Rocky Mountain Towards Forest and Range Experiment Station, Fort Collins, COGoogle Scholar
  30. 30.
    Ohno S, Muramoto J, Christian L, Atkin NB (1967) Diploid-tetraploid relationship among old-world members of the fish family Cyprinidae". Chromosoma 23:1–9CrossRefGoogle Scholar
  31. 31.
    Bao WK (1988) Polyploidy. In: Cai X (ed) Plant genetics and breeding. Science, Beijing, pp 611–624Google Scholar
  32. 32.
    Johnsson H (1944) Triploidy in Betula alba L. Bot Notiser Lund 1:85–96Google Scholar
  33. 33.
    Castillo A, Budak H, Varshney RK, Dorado G, Graner A, Hernandez P (2008) Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense. BMC Plant Biol. doi: 10.1186/1471-2229-8-97 PubMedGoogle Scholar
  34. 34.
    Hanley SJ, Mallott MD, Karp A (2006) Alignment of a Salix linkage map to the Populus genomic sequence reveals macrosynteny between willow and Populus genomes. Tree Genet Genomes 3:35–48CrossRefGoogle Scholar
  35. 35.
    Li YC, Korol AB, Beiles A et al (2002) Microsatellites: genomic distribution, putative functions and mutational mechanisms: a review. Mol Ecol 11:2453–2465PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Fanming Kong
    • 1
  • Jingjing Liu
    • 1
  • Yingnan Chen
    • 1
  • Zhibing Wan
    • 1
  • Tongming Yin
    • 1
    Email author
  1. 1.The Key Laboratory of Forest Genetics and BiotechnologyNanjing Forestry UniversityNanjingChina

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