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BioEnergy Research

, Volume 6, Issue 1, pp 1–13 | Cite as

Screening Microalgae Strains for Biodiesel Production: Lipid Productivity and Estimation of Fuel Quality Based on Fatty Acids Profiles as Selective Criteria

  • Iracema Andrade NascimentoEmail author
  • Sheyla Santa Izabel Marques
  • Iago Teles Dominguez Cabanelas
  • Solange Andrade Pereira
  • Janice Isabel Druzian
  • Carolina Oliveira de Souza
  • Daniele Vital Vich
  • Gilson Correia de Carvalho
  • Maurício Andrade Nascimento
Article

Abstract

The viability of algae-based biodiesel industry depends on the selection of adequate strains in regard to profitable yields and oil quality. This work aimed to bioprospecting and screening 12 microalgae strains by applying, as selective criteria, the volumetric lipid productivity and the fatty acid profiles, used for estimating the biodiesel fuel properties. Volumetric lipid productivity varied among strains from 22.61 to 204.91 mg l−1 day−1. The highest lipid yields were observed for Chlorella (204.91 mg l−1 day1) and Botryococcus strains (112.43 and 98.00 mg l−1 day−1 for Botryococcus braunii and Botryococcus terribilis, respectively). Cluster and principal components analysis analysis applied to fatty acid methyl esters (FAME) profiles discriminated three different microalgae groups according to their potential for biodiesel production. Kirchneriella lunaris, Ankistrodesmus fusiformis, Chlamydocapsa bacillus, and Ankistrodesmus falcatus showed the highest levels of polyunsaturated FAME, which incurs in the production of biodiesels with the lowest (42.47–50.52) cetane number (CN), the highest (101.33–136.97) iodine values (IV), and the lowest oxidation stability. The higher levels of saturated FAME in the oils of Chlamydomonas sp. and Scenedesmus obliquus indicated them as source of biodiesel with higher oxidation stability, higher CN (63.63–64.94), and lower IV (27.34–35.28). The third group, except for the Trebouxyophyceae strains that appeared in isolation, are composed by microalgae that generate biodiesel of intermediate values for CN, IV, and oxidation stability, related to their levels of saturated and monosaturated lipids. Thus, in this research, FAME profiling suggested that the best approach for generating a microalgae-biodiesel of top quality is by mixing the oils of distinct cell cultures.

Keywords

Biodiesel quality Fatty acid profiles Lipid productivity Microalgae bioprospection Microalgae selection for biodiesel production 

Abbreviations

ANP

Brazilian National Agency for Petroleum, Natural Gas and Biofuels

ASTM

American Society for Testing and Materials

CFPP

Cold filter plugging point

CN

Cetane number

DU

Degree of unsaturation

FA

Fatty acids

FAME

Fatty acid methyl esters

GHG

Greenhouse gases

IV

Iodine value

Lc

Total lipids content

LCSF

Long-chain saturated factor

Lp

Volumetric lipid productivity

MJ

Mega Joule

MUFA

Monounsaturated fatty acids

OD

Optical density

OS

Oxidative stability

Pdwt

Biomass productivity

PUFA

Polyunsaturated fatty acids

SV

Saponification value

TAG

Triglycerides

Notes

Acknowledgments

This work benefited from the support from the National Council for the Scientific and Technological Development (CNPQ)/Science and Technology Ministry, Brazil (processes 574712/2008-9 and 551134/2010-0).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Iracema Andrade Nascimento
    • 1
    • 5
    Email author
  • Sheyla Santa Izabel Marques
    • 1
  • Iago Teles Dominguez Cabanelas
    • 1
  • Solange Andrade Pereira
    • 1
  • Janice Isabel Druzian
    • 2
  • Carolina Oliveira de Souza
    • 2
  • Daniele Vital Vich
    • 1
  • Gilson Correia de Carvalho
    • 3
  • Maurício Andrade Nascimento
    • 4
  1. 1.Institute of BiologyFederal University of BahiaSalvadorBrazil
  2. 2.Institute of PharmacyFederal University of BahiaSalvadorBrazil
  3. 3.Health Sciences InstituteFederal University of BahiaSalvadorBrazil
  4. 4.Polytechnic InstituteFederal University of BahiaSalvadorBrazil
  5. 5.SalvadorBrazil

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