BioEnergy Research

, Volume 5, Issue 2, pp 287–293

Bioconversion of Butyric Acid to Butanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in a Limited Nutrient Medium

  • Najeeb Kaid Nasser Al-Shorgani
  • Ehsan Ali
  • Mohd Sahaid Kalil
  • Wan Mohtar Wan Yusoff
Article

Abstract

This study was designed to investigate the ability of Clostridium saccharoperbutylacetonicum N1-4 to produce butanol in a limited nutrient medium using mixtures of glucose and butyric acid as substrates. Specific combinations of glucose and butyric acid were found to influence the enhancement and retardation of butanol production as well as the reduction and modulation of the number of bacterial cells. Increasing the butyric acid concentration leads to the inhibition of bacterial growth, whereas the presence of (0–5 g/L) butyric acid and (0–10 g/L) glucose enhances the butanol production. The combination of 5 g/L butyric acid with 5 and 10 g/L of glucose was found to be the most suitable, but the use of glucose at concentrations greater than 10 g/L shifted the optimal butyric acid concentrations to 10 and 15 g/L for maximum butanol production signifying the requirement of a specific combination of glucose and butyric acid for enhanced butanol production in the fermentation process. C. saccharoperbutylacetonicum N1-4 demonstrated the ability to produce butanol in the absence of glucose, but no acetone or ethanol was produced under these conditions, reflecting the nature of the pathways involved in the production of butanol using only butyric acid. Ten grams per litre of butyric acid was found able to produce 13 g/L of butanol in the presence of 20 g/L of glucose, and 0.7 g/L butanol was produced in the absence of glucose. This study indicates the importance of the glucose to butyric acid ratio to the enhancement of butanol production.

Keywords

Butanol Clostridium saccharoperbutylacetonicum N1-4 Butyric acid bioconversion Limited nutrient medium Batch culture 

References

  1. 1.
    Andersch W, Bahl H, Gottschalk G (1983) Level of enzymes involved in acetate, butyrate, acetone and butanol formation by Clostridium acetobutylicum. Appl Microbiol Biotechnol 18:327–332CrossRefGoogle Scholar
  2. 2.
    Atsumi S, Hanai T, Liao JC (2008) Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature 451:86–90PubMedCrossRefGoogle Scholar
  3. 3.
    Dürre P (2007) Biobutanol: An attractive biofuel. Biotechnol J 2:1525–1534PubMedCrossRefGoogle Scholar
  4. 4.
    Ennis BM, Maddox IS (1985) Use of Clostridium acetobutylicum P262 for production of solvents from whey permeate. Biotechno Lett 7:601–606CrossRefGoogle Scholar
  5. 5.
    Fond O, Matta-Ammouri G, Petitdemange H, Engasser JM (1985) The role of acids on the production of acetone and butanol by Clostridium acetobutylicum. Appl Microbiol Biotechnol 22:195–200CrossRefGoogle Scholar
  6. 6.
    Gottschal JC, Morris JG (1981) The induction of acetone and butanol production in cultures of Clostridium acetobutylicum by elevated concentrations of acetate and butyrate. FEMS Microbiol Lett 12:385–389CrossRefGoogle Scholar
  7. 7.
    Harris LM, Desai RP, Welker NE, Papoutsakis ET (2000) Characterization of recombinant strains of the Clostridium acetobutylicum butyrate kinase inactivation mutant: need for new phenomenological models for solventogenesis and butanol inhibition? Biotechnol Bioeng 67:1–11PubMedCrossRefGoogle Scholar
  8. 8.
    Jones DT, Woods DR (1986) Acetone-butanol fermentation revisited. Microbiol Rev 50:484–524PubMedGoogle Scholar
  9. 9.
    Kalil MS, Saleha S, Yussof WMW (2006) Production of Acetone, Butanol and Ethanol (ABE) by Clostridium saccharoperbutylacetonicum N1-4 with different immobilization system. Pak J Biol Sci 9:1923–1928CrossRefGoogle Scholar
  10. 10.
    Madihah MS, Ariff AB, Sahaid KM, Suraini AA, Karim MIA (2001) Direct fermentation of gelatinized sago starch to acetone–butanol–ethanol by Clostridium acetobutylicum World. J Microbiol Biotechnol 17:567–576CrossRefGoogle Scholar
  11. 11.
    Martin JR, Petitdemange H, Ballongue J, Gay J (1983) Effects of acetic and butyric acids on solvents production by Clostridium acetobutylicum. Biotechnol Letters 5:89–94CrossRefGoogle Scholar
  12. 12.
    Monot F, Engasser JM, Petitdemange H (1984) Influence of pH and undissociated butyric acid on the production of acetone and butanol in batch cultures of Clostridium acetobutylicum. Appl Microbiol Biotechnol 19:422–426CrossRefGoogle Scholar
  13. 13.
    Nolasco H, Crabbe E, Badillo CM, Zarrabal OC, Mora MAM, Flores GP et al (2008) Bioconversion of industrial wastewater from palm oil processing to butanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). J Cleaner Production 16:632–638CrossRefGoogle Scholar
  14. 14.
    Qureshi N, Blaschek HP (2000) ABE production from corn: a recent economic evaluation. J Ind Microbiol Biotechnol 27:292–297CrossRefGoogle Scholar
  15. 15.
    Sang YL, Park J, Seh H, Lars KN, Jaehyun K, Kwang S (2008) Fermentative Butanol Production by Clostridia. J Biotechnol Bioeng 101:209–228CrossRefGoogle Scholar
  16. 16.
    Shinto H, Tashiro Y, Yamashita M, Kobayashi G, Sekiguchi T, Hanai T et al (2007) Kinetic modeling and sensitivity analysis of acetone-butanol-ethanol production. J Biotechnol 131:45–56PubMedCrossRefGoogle Scholar
  17. 17.
    Soni BK, Das K, Ghose TK (1987) Inhibitory factors involved in acetone-butanol fermentation by Clostridium sacchroperbutylacetonicum. Curr Microbiol 16:61–67CrossRefGoogle Scholar
  18. 18.
    Tashiro Y, Shinto H, Hayashi M, Baba KG, Sonomoto K (2007) Novel High-Efficient Butanol Production from Butyrate by Non-Growing Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) with Methyl Viologen. J Biosci Bioeng 104:238–240PubMedCrossRefGoogle Scholar
  19. 19.
    Terracciano JS, Kashket ER (1986) Intracellular conditions required for initiation of solvent production by Clostridium acetobutylicum. Appl Environ Microbiol 52:86–91PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Najeeb Kaid Nasser Al-Shorgani
    • 2
  • Ehsan Ali
    • 1
  • Mohd Sahaid Kalil
    • 1
  • Wan Mohtar Wan Yusoff
    • 2
  1. 1.Department of Chemical and Process Engineering, Faculty of EngineeringUniversiti Kebangsaan MalaysiaBangiMalaysia
  2. 2.School of Bioscience and Biotechnology, Faculty of Science and TechnologyUniversiti Kebangsaan MalaysiaBangiMalaysia

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