Journal of Chemical Biology

, Volume 6, Issue 2, pp 51–62 | Cite as

Synthetic circuit of inositol phosphorylceramide synthase in Leishmania: a chemical biology approach

  • Vineetha Mandlik
  • Dixita Limbachiya
  • Sonali Shinde
  • Milsee Mol
  • Shailza Singh
Short Communication
First Online:
Received:
Accepted:

Abstract

Building circuits and studying their behavior in cells is a major goal of systems and synthetic biology. Synthetic biology enables the precise control of cellular states for systems studies, the discovery of novel parts, control strategies, and interactions for the design of robust synthetic systems. To the best of our knowledge, there are no literature reports for the synthetic circuit construction for protozoan parasites. This paper describes the construction of genetic circuit for the targeted enzyme inositol phosphorylceramide synthase belonging to the protozoan parasite Leishmania. To explore the dynamic nature of the circuit designed, simulation was done followed by circuit validation by qualitative and quantitative approaches. The genetic circuit designed for inositol phosphorylceramide synthase (Biomodels Database—MODEL1208030000) shows responsiveness, oscillatory and bistable behavior, together with intrinsic robustness.

Keywords

IPCS Leishmania Genetic circuit Simulation Degradation rate Logic circuit 
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Notes

Acknowledgments

The authors would like to thank Dr. SC Mande, Director NCCS for supporting the Bioinformatics and High Performance Computing Facility. Vineetha Mandlik acknowledges the financial support as Junior Research Fellow of Department of Biotechnology, Government of India. The work was supported by the Department of Biotechnology, New Delhi, Govt. of India.

Supplementary material

12154_2012_89_MOESM1_ESM.docx (3.5 mb)
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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Vineetha Mandlik
    • 1
  • Dixita Limbachiya
    • 2
  • Sonali Shinde
    • 1
  • Milsee Mol
    • 1
  • Shailza Singh
    • 1
  1. 1.National Centre for Cell SciencePuneIndia
  2. 2.Department of Computer Science and TechnologySardar Patel UniversityVallabh VidyanagarIndia

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