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Annals of Nuclear Medicine

, Volume 23, Issue 4, pp 333–339 | Cite as

Bioevaluation of 90Y-labeled particles in animal model of arthritis

  • Usha Pandey
  • Ketaki Bapat
  • Haladhar Dev Sarma
  • Prem Singh Dhami
  • P. W. Naik
  • Grace Samuel
  • Meera VenkateshEmail author
Original Article

Abstract

Objective

The aim of the present study was to evaluate the behaviour of 90Y-labeled particles when injected into an arthritic knee joint of Wistar rats with severe inflammation induced using Complete Freund’s Adjuvant (CFA).

Methods

90Y-ferric hydroxide macroaggregates (90Y-FHMA), 90Y-hydroxyapatite (90Y-HA) and 90Y-phosphate particles (90YPO4) were prepared, subjected to quality control analyses and in vitro stability studies. Biodistribution studies were carried out by intra-articular injection into knee joints of Wistar rats induced with chronic inflammatory arthritis using CFA and by monitoring the radioactivity for retention and leakage.

Results

All the three preparations exhibited ~99% localization in knee joints even at 24 h p.i. with very small amounts observed in the liver and lungs, possibly due to leakage of the radiolabeled particles from the inflamed knee joint. Absence of any radioactivity in the femur indicated the in vivo stability of the particle preparations.

Conclusions

The biodistribution patterns were very similar in the normal and arthritic rats and were associated with negligible leakage (up to 24 h) from the knee joint indicating the potential of all the 90Y-radiolabeled preparations reported here, for use in radiation synoviorthesis.

Keywords

90Y-labeled particles Radiosynoviorthesis Complete Freund’s Adjuvant 

Notes

Acknowledgments

The authors express their sincere thanks to Dr. V. Venugopal, Director, Radiochemistry and Isotope Group for his encouragement and support.

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Copyright information

© The Japanese Society of Nuclear Medicine 2009

Authors and Affiliations

  • Usha Pandey
    • 1
  • Ketaki Bapat
    • 1
  • Haladhar Dev Sarma
    • 2
  • Prem Singh Dhami
    • 3
  • P. W. Naik
    • 3
  • Grace Samuel
    • 1
  • Meera Venkatesh
    • 1
    Email author
  1. 1.Radiopharmaceuticals DivisionBhabha Atomic Research CentreMumbaiIndia
  2. 2.Radiation Biology and Health Sciences DivisionBhabha Atomic Research CentreMumbaiIndia
  3. 3.Fuel Reprocessing DivisionBhabha Atomic Research CentreMumbaiIndia

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