FAIMS-MS-IR spectroscopy workflow: a multidimensional platform for the analysis of molecular isoforms
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An original workflow allowing inline FAIMS separation, electrospray ionization, mass analysis and ion spectroscopy (IRMPD: InfraRed Multiple Photon Dissociation) is presented for multidimensional molecular analysis. This new instrument consists of an ultraFAIMS (Owlstone) device interfaced to a linear ion trap (LTQ XL Thermo Scientific) which was modified for IRMPD spectroscopy. Two modes of operation are demonstrated on an isomeric mixture of paracetamol and 2-phenylglycine. In the first mode a FAIMS (high-Field Asymmetric waveform Ion Mobility Spectrometry) separation of the isomers is performed with a static compensation field for mass- and isomer- selective ion spectroscopy. In the second mode, the compensation field is scanned while the ions are irradiated at a fixed wavenumber. The advantages of this workflow as compared to traditional FAIMS-MS and IRMPD spectroscopy are described. The potential of the two modes for molecular spectroscopy and analytical applications, in particular the new “omics” are discussed.
KeywordsFAIMS Isomers Ion spectroscopy Multidimensional analysis Structural characterization
This work was supported by Institut Universitaire de France, ANR Circé (grant ANR-16-CE30-0012) the Fédération de Recherche André Marie Ampère and the Glycophysics Network (web: http://glyms.univ-lyon1.fr) funded by the French Agence Nationale de la Recherche (grant ANR-2015-MRSEI-0010).
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