Signals in human breath related to Sarcoidosis. — Results of a feasibility study using MCC/IMS

  • A. Bunkowski
  • B. Bödeker
  • S. Bader
  • M. Westhoff
  • P. Litterst
  • J. I. Baumbach
Original Research

Abstract

Using a 63Ni—Ion Mobility Spectrometer (IMS) coupled with a Multi-Capillary Column (MCC) the signals obtained are considered to identify characteristic peaks of volatile compounds in exhaled human breath samples of 10 mL volume. The breath of 20 patients with sarcoidosis and suspicion of sarcoidosis because of mediastinal lymp node enlargement was investigated. It could be shown that a procedure related to a single peak in the IMS-chromatogram delivers a differentiation into the two groups of patients with confirmed sarcoidosis and such suffering no sarcoidosis. The potential biomarker is characterised by the following parameters inverse mobility (1/K0) 0.53 ± 0.01 Vs/cm2—retention time 22 ± 5 s. These results are a first step in breath analysis by MCC/IMS in patients with sarcoidosis.

Keywords

Human breath MCC/IMS Sarcoidosis IMS-chromatogram VOC 

Notes

Acknowledgements

The authors wish to thank Mrs. Stefanie Güssgen, Mrs. Barbara Obertrifter, Mrs. Lucia Seifert, Mrs. Susanne Krois and Dr. Wolfgang Vautz for their major contributions to experimental and laboratory work. The use of data obtained during the work of Dr. Vera Ruzsanyi published partly in her PhD thesis and such of Martin Meier, obtained at his stay at ISAS and lung hospital Hemer during his diploma thesis should be mentioned with thanks.

The financial support of the Bundesministerium für Bildung und Forschung and the Ministerium für Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen is gratefully acknowledged. The work was founded partly by the project BAMOD (Breath-gas analysis for molecular-oriented detection of minimal diseases) of the European Union (LSHC-CT-2005-019031) and the high-tech strategy funds of the Federal Republic of Germany (project Metabolit—01SF0716).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • A. Bunkowski
    • 1
  • B. Bödeker
    • 1
    • 2
  • S. Bader
    • 1
    • 3
  • M. Westhoff
    • 4
  • P. Litterst
    • 4
  • J. I. Baumbach
    • 1
  1. 1.Metabolomics DepartmentISAS — Institute for Analytical SciencesDortmundGermany
  2. 2.B & S Analytik GmbHDortmundGermany
  3. 3.Roche Diagnistik GmbHPenzbergGermany
  4. 4.Lung Hospital HemerHemerGermany

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