Exemplar application of multi-capillary column ion mobility spectrometry for biological and medical purpose

Original Research
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Abstract

In recent years, ion mobility spectrometry is increasingly in demand for new applications especially on biological samples (cells, bacteria, fungi), in medicine (diagnosis, therapy and medication control e.g. from breath analyses), for food quality control, safety monitoring and characterisation or process control in chemical and pharmaceutical industry. For this purpose instruments based on gas phase separation of ions in weak electric fields were developed at ISAS–Institute for Analytical Sciences, focussing on the particular challenges such as humid and rather complex samples, specific sampling procedures adapted to the application, fast pre-separation techniques like multi-capillary columns and suitable data processing including data bases for relevant analytes and automatic characterisation of IMS-chromatograms. Feasibility studies were carried out successfully for biological and medical purpose at ISAS, including the detection of bacteria, fungi and metabolites of cells and in human breath. For all those samples characteristic pattern of analytes were found and could be used for the identification of cell lines, fungi and bacteria as well as of numerous diseases. Furthermore, the quantification of those analytes could be used to obtain information about the state of the process or person (e.g. growth of cultures, development of diseases, level of medication, grade of cancer). Those examples shall demonstrate the potential of ion mobility spectrometry for the selected applications. However, a general and reliable data bases of reference analytes is required in the near future to enable an exploitation of the metabolic pathways and to confirm the relevance of the detected signals for the investigated topic.

Keywords

Ion mobility spectrometry Trace gas analysis Metabolites Metabolic profiling Metabolomics Cells Fungi Bacteria Breath analysis Diseases Medication Therapy 
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Notes

Acknowledgement

The financial support of the Bundesministerium für Bildung und Forschung and the Ministerium für Wissenschaft und Forschung des Landes Nordrhein-Westfalen is gratefully acknowledged. The co-operation with the Lung Hospital in Hemer, Germany, in particular Dr. Lutz Freitag, Dr. Michael Westhoff and Dr. Patrick Litterst and the participation of all the healthy subjects and patients was essential for the breath analyses investigations. The bacteria and fungi investigations were carried out at the Hygiene-Institut in Iserlohn, Germany. The dedicated work of Luzia Seifert and Susanne Krois, both technicians at ISAS, was indispensable for the success of the investigations as well as the support of Dunja Zimmermann and Michéle Hartmann during the work with the cell lines and of Dr. Jürgen Nolte and Rita Fobbe for the identification of unknown analytes using mass spectrometry. Last but not least, the contributions of Sabine Bader related to data processing and evaluation potentiated the interpretation of the numerous and complex data sets.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.ISAS–Institute for Analytical SciencesDortmundGermany

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