Head and Neck Pathology

, Volume 2, Issue 3, pp 169–174 | Cite as

Human Kallikrein 8 Expression in Salivary Gland Tumors

  • Mark R. Darling
  • Sam Tsai
  • Linda Jackson-Boeters
  • Thomas D. Daley
  • Eleftherios P. Diamandis
Original Paper
First Online:
Received:
Accepted:

Abstract

The human kallikrein 8 protein (KLK8) is expressed in many normal tissues including esophagus, skin, testis, tonsil, kidney, breast, and salivary gland, and is found in biological fluids including breast milk, amniotic fluid, seminal fluid and serum. It has also been shown to be a biomarker and prognostic factor for breast cancer. The aim of this study was to determine whether KLK8 is expressed in salivary gland tissues and salivary gland tumors (both benign and malignant), in order to compare normal with tumor tissues. Pleomorphic adenomas, adenoid cystic carcinomas, polymorphous low grade adenocarcinomas, acinic cell carcinomas, mucoepidermoid carcinomas, and adenocarcinomas NOS of both minor and major salivary glands were examined. The results of this study indicate that most salivary gland tumors show high levels of expression of KLK8.

Keywords

Kallikreins Human kallikrein 8 Salivary gland tumors Prognostic markers Immunohistochemistry 

Abbreviations

PA

Pleomorphic adenoma

ACC

Adenoid cystic carcinoma

PLGA

Polymorphous low grade adenocarcinoma

ACI

Acinic cell carcinoma

MEC

Mucoepidermoid carcinoma

ANOS

Adenocarcinoma not otherwise specified

KLK

Human kallikrein protein

KLK

Human kallikrein gene

RT

Room temperature

PBS

Phosphate-buffered saline

Pab

Polyclonal antibody

DAB

3,3′-Diaminobenzidine

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Notes

Acknowledgments

The authors are grateful to Linda Grass for technical advice and to the National Organization for Rare Disorders which provided funding for this study.

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Copyright information

© Humana 2008

Authors and Affiliations

  • Mark R. Darling
    • 1
  • Sam Tsai
    • 2
  • Linda Jackson-Boeters
    • 1
  • Thomas D. Daley
    • 1
  • Eleftherios P. Diamandis
    • 3
    • 4
  1. 1.Division of Oral Pathology, Department of PathologyUniversity of Western OntarioLondonCanada
  2. 2.Faculty of Medicine & DentistryUniversity of AlbertaEdmontonCanada
  3. 3.Department of Pathology and Laboratory MedicineMount Sinai HospitalTorontoCanada
  4. 4.Department of Laboratory Medicine and PathobiologyUniversity of TorontoTorontoCanada

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