Resonance assignments of bacteriophage SPO1 Gp49 protein

  • Zhihao Wang
  • Yingzi Liang
  • Hui Liu
  • Yawen Wang
  • Hongliang Wang
  • Bing LiuEmail author


Recent applications of phage therapy in localized wound and drug-resistant bacterial infection have brought bacteriophage back to the spotlight. While these works demonstrated the safety and effectiveness of engineered bacteriophages in human patients, the exact molecular machinery behind the bacteria killing remains largely uncharacterized. This is particularly noticable outside Escherichia coli phages, as most studies are based on bacteriophages of this Gram-negative model bacterium. In the attempt to extent our understanding to the bacteriophage of Gram-positive bacteria, we chose the host hijacking module of Bacillus subtilis phage SPO1 for systemic functional and structural studies. Gp49, an acidic protein located within operon 4 of this module, is believed to have a role during the host takeover event. Here we describe the complete resonance assignment of Gp49, which shares no sequence homology with any known protein, as the basis for the structure determination and further mechanism study.


SPO1 Bacteriophage Bacillus subtilis Gp49 Host takeover module 



We thank Professor Steve Matthews of Imperial College London for useful discssions. We also thank Dr Zhijun Liu and Mr. Wei Zhan of Zhangjiang Laboratory, Chinese Academy of Sciences for recording spectra on 900 MHz spectrometer.


This research is supported by Young Talent project of Xi’an Jiaotong University for Professor Bing Liu and special equipment fund from Xi’an Jiaotong University 1st affiliated Hospital.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Brodetsky AM, Romig WR (1965) Characterization of Bacillus subtilis bacteriophages. J Bacteriol 90:1663CrossRefGoogle Scholar
  2. Dedrick RM et al (2019) Engineered bacteriophages for treatment of a patient with a disseminated drug-resistant Mycobacterium abscessus Nat Med 25:733. CrossRefGoogle Scholar
  3. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:293. CrossRefGoogle Scholar
  4. Fillol-Salom A et al (2019) Hijacking the hijackers: Escherichia coli pathogenicity islands redirect helper phage packaging for their own benefit. Mol Cell 75:1030 e1024. CrossRefGoogle Scholar
  5. Johnson BA, Blevins RA (1994) NMR view: a computer program for the visualization and analysis of NMR data. J Biomol NMR 4:614. CrossRefGoogle Scholar
  6. Mulvenna N, Hantke I, Burchell L, Nicod S, Bell D, Turgay K, Wigneshweraraj S (2019) Xenogeneic modulation of the ClpCP protease of Bacillus subtilis by a phage-encoded adaptor-like protein. J Biol Chem. CrossRefGoogle Scholar
  7. Salmond GP, Fineran PC (2015) A century of the phage: past, present and future. Nat Rev Microbiol 13:786. CrossRefGoogle Scholar
  8. Sampath A, Stewart CR (2004) Roles of genes 44, 50, and 51 in regulating gene expression and host takeover during infection of Bacillus subtilis by bacteriophage SPO1. J Bacteriol 186:1792. CrossRefGoogle Scholar
  9. Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44:223. CrossRefGoogle Scholar
  10. Shi J, Wen A, Zhao M, You L, Zhang Y, Feng Y (2019) Structural basis of sigma appropriation. Nucleic Acids Res 47:9432. CrossRefGoogle Scholar
  11. Stewart CR et al (1998) Genes and regulatory sites of the "host-takeover module" in the terminal redundancy of Bacillus subtilis bacteriophage SPO. Virology 1:340. CrossRefGoogle Scholar
  12. Stewart CR et al (2009) The genome of Bacillus subtilis bacteriophage SPO1. J Mol Biol 388:70. CrossRefGoogle Scholar
  13. Tabib-Salazar A et al (2017) Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein. Nucleic Acids Res 45:7707. CrossRefGoogle Scholar
  14. Wu CH, Liu IJ, Lu RM, Wu HC (2016) Advancement and applications of peptide phage display technology in biomedical science. J Biomed Sci 23:8. CrossRefGoogle Scholar
  15. Zhang P, Wang Z, Zhao S, Wang Y, Matthews S, Liu B (2019) (1)H, (13)C and (15)N NMR assignments of Bacillus subtilis bacteriophage SPO1 protein Gp46. Biomol NMR Assign 13:247. CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2020

Authors and Affiliations

  1. 1.BioBank, First Affiliated Hospital, School of MedicineXi’an Jiaotong UniversityXi’anChina
  2. 2.Department of Pathogen Biology and Immunology, School of Basic Medical SciencesXi’an Jiaotong UniversityXi’anChina
  3. 3.Instrumental Analysis Center of Xi’an Jiaotong UniversityXi’anChina

Personalised recommendations