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Backbone assignment of cytochrome PccH, a crucial protein for microbial electrosynthesis in Geobacter sulfurreducens

Abstract

Microbial electrosynthesis is an emerging green technology that explores the capability of a particular group of microorganisms to drive their metabolism toward the production of hydrogen or value-added chemicals from electrons supplied by electrode surfaces. The cytochrome PccH showed the largest increase in transcription when electrons are supplied to Geobacter sulfurreducens biofilms. Gene knock-out experiments have shown that the electron transfer toward G. sulfurreducens cells was completely inhibited by the deletion of the gene encoding for cytochrome PccH. This identifies a crucial role for this protein in G. sulfurreducens microbial electrosynthesis mechanisms, which are currently unknown. In this work, we present the backbone (1H, 13C and 15N) and heme assignment for PccH in the oxidized state. The data obtained paves the way to identify and structurally map the molecular interaction regions between the cytochrome PccH and its physiological redox partners.

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Acknowledgements

This work was supported by the following Fundação para a Ciência e a Tecnologia (FCT-MCTES) Grants: PTDC/BIA-BQM/31981/2017 (to CAS) and scholarship Grant No. PD/BD/114445/2016 (to LRT) through Radiation Biology and Biophysics Doctoral Training Programme (RaBBiT, PD/00193/2012, UID/FIS/00068/2013 (CEFITEC). This work was also supported by Unidade de Ciências Biomoleculares Aplicadas-UCIBIO which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). We acknowledge the use of the “Manuel Rico” NMR laboratory, LMR (CSIC), a Spanish large-scale national NMR facility ICTS R-LRB.

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Correspondence to Marta Bruix or Carlos A. Salgueiro.

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Teixeira, L.R., Portela, P.C., Morgado, L. et al. Backbone assignment of cytochrome PccH, a crucial protein for microbial electrosynthesis in Geobacter sulfurreducens. Biomol NMR Assign 13, 321–326 (2019). https://doi.org/10.1007/s12104-019-09899-6

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Keywords

  • Microbial electrosynthesis
  • Geobacter sulfurreducens
  • Cytochrome
  • PccH
  • NMR