Backbone and side-chain resonance assignments of centromeric protein Scm3 from Saccharomyces cerevisiae
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The centromeric chromatin plays an essential role in regulating the attachment of microtubules and controlling the segregation of sister chromatids during mitosis. In budding yeast, the evolutionary conserved histone variant, Cse4 is a vital component of the multiprotein kinetochore complex and is recruited to the centromere through its chaperone, Suppressor of chromosome mis-segregation (Scm3). Scm3 is an inner kinetochore protein crucial for the formation of a functional inner kinetochore. Scm3 has been known to play an active role in the assembly of the centromeric nucleosome and its deletion has been found to have deleterious effects on the cells leading to chromosome segregation defects. However, structural details of monomeric full length Scm3 have remained elusive so far. Here, we report the backbone and side-chain resonance assignments of centromeric protein, Scm3. 1H, 13C and 15N chemical shifts of Scm3 have been obtained by various 2D and 3D heteronuclear NMR experiments at pH 7.4 and 283 K.
KeywordsNMR Resonance assignments Centromeric protein Scm3 MUSIC
We are thankful to RIFC, IRCC, NMR facility (750 MHz) at IIT Bombay. We also thank Dr. Veera Mohan Rao Kakita for helping us in the recording of NMR spectra. AB is the recipient of a research fellowship from the Department of Atomic Energy, Mumbai, India, and VKS (PDF/2016/000255) is thankful to the Department of Science and Technology-Science and Engineering Research Board, Govt. of India, New Delhi for the award of National Post-Doctoral Fellowships. AK is thankful to the Department of Biotechnology, Govt. of India for financial support (Grant No. BT/PR21656/BRB/10/1562/2016).
- Keller R (2004) The computer aided resonance assignment tutorial CANTINA. Verlag, GoldauGoogle Scholar