Biomolecular NMR Assignments

, Volume 13, Issue 1, pp 177–181 | Cite as

Resonance assignments for the tandem PWWP-ARID domains of human RBBP1

  • Weibin Gong
  • Xingzhe Yao
  • Qihui Liang
  • Yufeng Tong
  • Sarah PerrettEmail author
  • Yingang FengEmail author


Retinoblastoma-binding protein 1 (RBBP1), also known as AT-rich interaction domain 4A (ARID4A), is a tumour suppressor involved in the regulation of the epigenetic programming in leukemia and Prader-Willi/Angelman syndromes. The ARID domain of RBBP1 binds to DNA non-specifically and has gene suppression activity. However, no structural data has been obtained for the human RBBP1 ARID domain so far. Here we report the near-complete 1H, 13C, 15N backbone and side-chain NMR assignment of a 27 kDa tandem PWWP-ARID domain construct that spans residues 171–414 with the removal of a short disordered region between the two domains. The predicted secondary structure based on the assigned chemical shifts is consistent with the structures of the isolated PWWP domain of human RBBP1 previously solved and the homologous ARID domains of other proteins.


RBBP1 ARID domain Tandem domains NMR assignments Secondary structure 



This work was support by a National Key R&D Program of China Grant 2017YFA0504000 to SP; National Natural Science Foundation of China Grants 31470747 to WG, 31670735 and 31661143023 to YF; a Beijing Natural Science Foundation Grant 5172026 to WG; and the CAS Center for Excellence in Biomacromolecules.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of BiophysicsChinese Academy of SciencesBeijingChina
  2. 2.Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina
  3. 3.CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina
  4. 4.Department of Chemistry & BiochemistryUniversity of WindsorWindsorCanada
  5. 5.University of the Chinese Academy of SciencesBeijingChina

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