1H, 15N, 13C resonance assignment of plant dehydrin early response to dehydration 10 (ERD10)
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Early response to dehydration 10 protein (ERD10) is an intrinsically disordered protein from Arabidopsis thaliana. The protein is upregulated during stress however its mechanism of action at atomic level is not well understood. In the present work multidimensional NMR methodologies are used in order to facilitate the process of chemical shift assignment. The information provided here supports further NMR spectroscopy experiments aimed at elucidation of ERD10 behaviour during molecular recognition events with other proteins.
KeywordsNMR Intrinsically disordered proteins Dehydrins ERD10 Multidimensional NMR spectrosocopy
CC and PT would like to thank Dr. Kris Pauwels for his critical assessment of this manuscript. CC was supported by the Marie Curie Initial Training Network project 264257 (IDPbyNMR). PT was supported by the Odysseus Grant G.0029.12 from Research Foundation Flanders (FWO).
CC and SZ contributed equally performing experiments and writing the article.
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Conflict of interest
No conflict of interest is manifested.
- Goddard T, Kneller D (2008) SPARKY 3. University of California, San Francisco. https://www.cgl.ucsf.edu/home/sparky/