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Biomolecular NMR Assignments

, Volume 10, Issue 2, pp 329–333 | Cite as

1H, 13C, and 15N resonance assignments for the pro-inflammatory cytokine interleukin-36α

  • Nishit Goradia
  • Amelie Wißbrock
  • Christoph Wiedemann
  • Frank Bordusa
  • Ramadurai Ramachandran
  • Diana Imhof
  • Oliver Ohlenschläger
Article

Abstract

Interleukin-36α (IL-36α) is a recently characterised member of the interleukin-1 superfamily. It is involved in the pathogenesis of inflammatory arthritis in one third of psoriasis patients. By binding of IL-36α to its receptor IL-36R via the NF-κB pathway other cytokines involved in inflammatory and apoptotic cascade are activated. The efficacy of complex formation is controlled by N-terminal processing. To obtain a more detailed view on the structure function relationship we performed a heteronuclear multidimensional NMR investigation and here report the 1H, 13C, and 15N resonance assignments for the backbone and side chain nuclei of the pro-inflammatory cytokine interleukin-36α.

Keywords

Backbone resonance assignments Heteronuclear NMR Interleukin-36α Interleukin-1 superfamily Cytokine 

Notes

Acknowledgments

Financial support by the Deutsche Forschungsgemeinschaft (DFG) within FOR 1738 (to D.I. and O.O.) is gratefully acknowledged. This work was partially supported by the BMBF network ProNet-T3 “Protein competence center Halle: tools, targets & therapeutics”. The FLI is a member of the Leibniz Association (WGL) and is financially supported by the Federal Government of Germany and the State of Thuringia.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Nishit Goradia
    • 1
  • Amelie Wißbrock
    • 3
  • Christoph Wiedemann
    • 2
  • Frank Bordusa
    • 2
  • Ramadurai Ramachandran
    • 1
  • Diana Imhof
    • 3
  • Oliver Ohlenschläger
    • 1
  1. 1.Leibniz Institute on Aging – Fritz Lipmann InstituteJenaGermany
  2. 2.Institute of Biochemistry/BiotechnologyMartin Luther University Halle-WittenbergHalle/SaaleGermany
  3. 3.Pharmaceutical Chemistry I, Institute of PharmacyUniversity of BonnBonnGermany

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