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Biomolecular NMR Assignments

, Volume 10, Issue 2, pp 325–328 | Cite as

Chemical shift assignments and secondary structure prediction for Q4DY78, a conserved kinetoplastid-specific protein from Trypanosoma cruzi

  • Éverton Dias D’Andréa
  • Anne Diehl
  • Peter Schmieder
  • Hartmut Oschkinat
  • José Ricardo Pires
Article
  • 229 Downloads

Abstract

Trypanosoma cruzi, Trypanosma brucei and Leishmania spp. are kinetoplastid protozoa causative agents of Chagas disease, sleeping sickness and leishmaniasis, respectively, neglected tropical diseases estimated to infect millions of people worldwide. Their genome sequencing has revealed approximately 50 % of genes encoding hypothetical proteins of unknown function, opening possibilities for novel target identification and drug discovery. Q4DY78 is a putative essential protein from T. cruzi conserved in the related kinetoplastids and divergent from mammalian host proteins. Here we report the 1H, 15N, and 13C chemical shift assignments and secondary structure analysis of the Q4DY78 protein as basis for NMR structure determination, functional analysis and drug screening.

Keywords

Hypothetical protein Q4DY78 Trypanosoma cruzi Kinetoplastids Neglected diseases NMR assignments 

Notes

Acknowledgments

The authors acknowledge the financial support obtained from the Brazilian Funding Agencies CNPq, CAPES (Process Number 99999.011648/2013-09) and FAPERJ.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Éverton Dias D’Andréa
    • 1
  • Anne Diehl
    • 2
  • Peter Schmieder
    • 2
  • Hartmut Oschkinat
    • 2
  • José Ricardo Pires
    • 1
  1. 1.Instituto de Bioquímica Médica Leopoldo de MeisUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Leibniz-Institut für Molekulare PharmakologieBerlinGermany

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