Biomolecular NMR Assignments

, Volume 10, Issue 2, pp 277–280 | Cite as

Backbone and side-chain 1H, 13C, and 15N chemical shift assignments for the apo-form of the lytic polysaccharide monooxygenase NcLPMO9C

  • Gaston Courtade
  • Reinhard Wimmer
  • Maria Dimarogona
  • Mats Sandgren
  • Vincent G. H. Eijsink
  • Finn L. Aachmann
Article

Abstract

The apo-form of the 23.3 kDa catalytic domain of the AA9 family lytic polysaccharide monooxygenase NcLPMO9C from Neurospora crassa has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the 1H, 13C, and 15N chemical shift assignments of this LPMO.

Keywords

Lytic polysaccharide monooxygenase LPMO AA9 Cellulose Xyloglucan 

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Gaston Courtade
    • 1
  • Reinhard Wimmer
    • 2
  • Maria Dimarogona
    • 3
  • Mats Sandgren
    • 3
  • Vincent G. H. Eijsink
    • 4
  • Finn L. Aachmann
    • 1
  1. 1.NOBIPOL, Department of BiotechnologyNorwegian University of Science and Technology (NTNU)TrondheimNorway
  2. 2.Department of Chemistry and BioscienceAalborg UniversityAalborg ØDenmark
  3. 3.Department of Chemistry and BiotechnologySwedish University of Agricultural SciencesUppsalaSweden
  4. 4.Department of Chemistry, Biotechnology and Food ScienceNorwegian University of Life SciencesÅsNorway

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