1H, 13C and 15N resonance assignments of human DCL-1 (CD302) extracellular domain
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DCL-1 (CD302) is a single-pass type one transmembrane protein which is predominantly expressed on myeloid cell lines. It possess the ability of endocytosis and is assumed to play a role in cell adhesion and migration. It has been also connected to several illnesses but more on the level of mRNA than on the protein expression level. More interestingly it is alternatively expressed in the form of a fusion protein with another single-pass type one transmembrane protein DEC205 (CD205) which is normally involved in antigen-uptake and endocytosis. The fusion protein has been assigned to have altered function compared to the wild type proteins. We have performed NMR structural analysis of the 16.2 kDa extracellular domain of DCL-1 to get a better insight onto this molecule. We have been able to assign nearly 97 % of resonance frequencies for the 15N and 13C labeled recombinant protein. The assignments have been deposited into Biological Magnetic Resonance Data Bank under the accession number 25802.
KeywordsDCL-1 (CD302) C-type lectin like receptor Protein NMR Heteronuclear NMR spectroscopy Resonance assignment
This work has been supported by the Institutional Research Concept of the Institute of Microbiology (RVO61388971); and grants from the Ministry of Education Youth and Sports of the Czech Republic (LO1509) and European Regional Development Funds (CZ.1.07/2.3.00/20.0055, CZ.1.07/2.3.00/30.0003 and CZ.1.05/1.1.00/02.0109); Charles University in Prague (GAUK 797213 and project UNCE 204025/2012); and the Czech Science Foundation (P207/10/1040). Access to instruments and other facilities was supported by the EU (Operational Program Prague—Competitiveness project CZ.2.16/3.1.00/24023).
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Conflict of interest
The authors declare that they have no conflict of interest.
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