NMR resonance assignments for the class II GTP binding RNA aptamer in complex with GTP
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The structures of RNA-aptamer-ligand complexes solved in the last two decades were instrumental in realizing the amazing potential of RNA for forming complex tertiary structures and for molecular recognition of small molecules. For GTP as ligand the sequences and secondary structures for multiple families of aptamers were reported which differ widely in their structural complexity, ligand affinity and ligand functional groups involved in RNA-binding. However, for only one of these families the structure of the GTP-RNA complex was solved. In order to gain further insights into the variability of ligand recognition modes we are currently determining the structure of another GTP-aptamer—the so-called class II aptamer—bound to GTP using NMR-spectroscopy in solution. As a prerequisite for a full structure determination, we report here 1H, 13C, 15N and partial 31P-NMR resonance assignments for the class II GTP-aptamer bound to GTP.
KeywordsRNA NMR-assignment Triple resonance experiments Aptamer GTP Selective labeling
We are grateful to Christian Richter and Manfred Strupf for maintenance of the NMR facility and to Heiko Keller and Kerstin Yacoub for providing the T7-RNA-polymerase. This work was supported by the Center for Biomolecular Magnetic Resonance (BMRZ) of the Johann-Wolfgang-Goethe-University Frankfurt and the Deutsche Forschungsgemeinschaft (DFG) (Grant WO 901/1-1).
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