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Biomolecular NMR Assignments

, Volume 10, Issue 1, pp 79–83 | Cite as

Backbone and side-chain resonance assignment of the A147T polymorph of mouse TSPO in complex with a high-affinity radioligand

  • Mariusz Jaremko
  • Łukasz Jaremko
  • Karin Giller
  • Stefan Becker
  • Markus ZweckstetterEmail author
Article

Abstract

The integral polytopic membrane protein TSPO is the target for numerous endogenous and synthetic ligands. However, the affinity of many ligands is influenced by a common polymorphism in TSPO, in which an alanine at position 147 is replaced by threonine, thereby complicating the use of several radioligands for clinical diagnosis. In contrast, the best-characterized TSPO ligand (R)-PK11195 binds with similar affinity to both variants of mitochondrial TSPO (wild-type and A147T variant). Here we report the 1H, 13C, 15N backbone and side-chain resonance assignment of the A147T polymorph of TSPO from Mus Musculus in complex with (R)-PK11195 in DPC detergent micelles. More than 90 % of all resonances were sequence-specifically assigned, demonstrating the ability to obtain high-quality spectral data for both the backbone and the side-chains of medically relevant integral membrane proteins.

Keywords

TSPO Polymorphism (R)-PK11195 NMR spectroscopy Membrane protein 

Notes

Acknowledgments

1H, 13C, 15N resonance assignments of the A147T-mTSPO/PK11195 complex have been deposited at the BMRB data base (Accession Code: 25513). This work was partially supported by the DFG Collaborative Research Center 803, Project A11.

Compliance with ethical standards

Conflict of interest

No conflict declared.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Mariusz Jaremko
    • 1
  • Łukasz Jaremko
    • 1
    • 2
  • Karin Giller
    • 1
  • Stefan Becker
    • 1
  • Markus Zweckstetter
    • 1
    • 2
    • 3
    Email author
  1. 1.Max-Planck-Institut für Biophysikalische ChemieGöttingenGermany
  2. 2.Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)GöttingenGermany
  3. 3.Center for Nanoscale Microscopy and Molecular Physiology of the BrainUniversity Medical CenterGöttingenGermany

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