Biomolecular NMR Assignments

, Volume 9, Issue 2, pp 435–440 | Cite as

NMR assignment of intrinsically disordered self-processing module of the FrpC protein of Neisseria meningitidis

  • Vojtěch Kubáň
  • Jiří Nováček
  • Ladislav Bumba
  • Lukáš ŽídekEmail author


The self-processing module (SPM) is an internal segment of the FrpC protein (P415–F591) secreted by the pathogenic Gram-negative bacterium Neisseria meningitidis during meningococcal infection of human upper respiratory tract. SPM mediates ‘protein trans-splicing’, a unique natural mechanism for editing of proteins, which involves a calcium-dependent autocatalytic cleavage of the peptide bond between D414 and P415 and covalent linkage of the cleaved fragment through its carboxy-terminal group of D414 to \(\epsilon\)-amino group of lysine residue within a neighboring polypeptide chain. We present an NMR resonance assignment of the calcium-free SPM, which displays characteristic features of intrinsically disordered proteins. Non-uniformly sampled 5D HN(CA)CONH, 4D HCBCACON, and HCBCANCO spectra were recorded to resolve poorly dispersed resonance frequencies of the disordered protein and 91 % of SPM residues were unambiguously assigned. Analysis of the chemical shifts revealed that two regions of the intrinsically disordered SPM (A95–S101 and R120–I127) have a tendency to form a helical structure, whereas the residues P1–D7 and G36–A40 have the propensity to adopt a \(\beta\)-structure.


FrpC Self-processing module Neisseria meningitidis Intrinsically disordered proteins Sparse sampling Resolution-enhanced spectroscopy Resonance assignment 



The authors thank Iva Maršíková for technical help with protein purifications. This work was supported by the project GAP207-11-0717 of the Grant Agency of the Czech Republic. Additional thanks go to CEITEC—the Central European Institute of Technology with research infrastructure supported by the Project CZ.1.05/1.1.00/02.0068 financed from the European Regional Development Fund.

Ethical standard

The authors declare that the experiments comply with the current laws of the Czech Republic.

Conflicts of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Vojtěch Kubáň
    • 1
  • Jiří Nováček
    • 1
  • Ladislav Bumba
    • 2
  • Lukáš Žídek
    • 1
    Email author
  1. 1.CEITECMasaryk UniversityBrnoCzech Republic
  2. 2.Institute of Microbiology of the ASCR, v. v. iPrague 4Czech Republic

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