Biomolecular NMR Assignments

, Volume 9, Issue 2, pp 333–336 | Cite as

NMR assignments for the insertion domain of bacteriophage CUS-3 coat protein

  • Therese N. Tripler
  • Mark W. Maciejewski
  • Carolyn M. TeschkeEmail author
  • Andrei T. AlexandrescuEmail author


CUS-3 is a P22-like tailed dsDNA bacteriophage that infects Escherichia coli serotype K1. The CUS-3 coat protein, which forms the icosahedral capsid, has a conserved HK97-fold but with a non-conserved accessory domain known as the insertion domain (I-domain). Sequence alignment of the coat proteins from CUS-3 and P22 shows higher sequence similarity for the I-domains (35 %) than for the HK97-cores, suggesting the I-domains play important functional roles. The I-domain of the P22 coat protein, which has an NMR structure comprised of a six-stranded β-barrel, has been shown to govern the assembly, stability and size of the resulting capsid particles. Here, we report the 1H, 15N, and 13C assignments for the I-domain from the coat protein of bacteriophage CUS-3. The secondary structure and dynamics of the CUS-3 I-domain, predicted from the assigned NMR chemical shifts, agree with those of the P22 I-domain, suggesting the CUS-3 and P22 I-domains may have similar structures and functions in capsid assembly.


Bacteriophage I-domain CUS-3 Viral assembly Procapsid 



We wish to thank Dr. K. Parent for supplying CUS-3 bacteriophage and Drs. A.D. Shuyker, M.R. Gryk, and J.C. Hoch for the use of the software platform NMRbox,, version 0.4a. This work was supported by NIH grant R01 GM076661 to C.M.T. and a supplement to C.M.T. and A.T.A.

Conflict of interest

The authors declare no conflict of interest.

Ethical standards

All experiments complied with all laws of the United States of America.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Department of Molecular & Cell Biology, and ChemistryUniversity of ConnecticutStorrsUSA
  2. 2.Department of Molecular, Microbial, and Structural BiologyUniversity of Connecticut HealthFarmingtonUSA
  3. 3.Department of ChemistryUniversity of ConnecticutStorrsUSA

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