Biomolecular NMR Assignments

, Volume 9, Issue 1, pp 135–138 | Cite as

Polypeptide backbone, Cβ and methyl group resonance assignments of the 24 kDa plectin repeat domain 6 from human protein plectin

  • Surya V. S. R. K. Pulavarti
  • Alexander Eletsky
  • Yuanpeng J. Huang
  • Thomas B. Acton
  • Rong Xiao
  • John K. Everett
  • Gaetano T. Montelione
  • Thomas Szyperski
Article
  • 109 Downloads

Abstract

The 500 kDa protein plectin is essential for the cytoskeletal organization of most mammalian cells and it is up-regulated in some types of cancer. Here, we report nearly complete sequence-specific polypeptide backbone, 13Cβ and methyl group resonance assignments for 24 kDa human plectin(4403–4606) containing the C-terminal plectin repeat domain 6.

Keywords

Cytoskeletal linker protein HCPIN Plakin repeat domain Plectin repeat domain Selective isotope labeling Structural genomics 

Notes

Acknowledgments

This work was supported by the National Institutes of Health, grant number: U54 GM094597 (T.S. and G.T.M.).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Surya V. S. R. K. Pulavarti
    • 1
    • 2
  • Alexander Eletsky
    • 1
    • 2
  • Yuanpeng J. Huang
    • 3
    • 4
  • Thomas B. Acton
    • 3
    • 4
  • Rong Xiao
    • 3
    • 4
  • John K. Everett
    • 3
    • 4
  • Gaetano T. Montelione
    • 3
    • 4
    • 5
  • Thomas Szyperski
    • 1
    • 2
  1. 1.Department of ChemistryThe State University of New York at BuffaloBuffaloUSA
  2. 2.Northeast Structural Genomics ConsortiumBuffaloUSA
  3. 3.Center for Advanced Biotechnology and Medicine and Department of Molecular Biology and BiochemistryRutgers, The State University of New JerseyPiscatawayUSA
  4. 4.Northeast Structural Genomics ConsortiumPiscatawayUSA
  5. 5.Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical SchoolRutgers, The State University of New JerseyPiscatawayUSA

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