Biomolecular NMR Assignments

, Volume 8, Issue 2, pp 357–360 | Cite as

1H, 15N and 13C chemical shift assignments for the winged helix domains of two archeal MCM C-termini

  • Christoph WiedemannEmail author
  • Oliver Ohlenschläger
  • Barbara Medagli
  • Silvia Onesti
  • Matthias Görlach


High-fidelity replication guarantees the stable inheritance of genetic information stored in the DNA of living organisms. The minichromosome maintenance (MCM) complex functions as replicative DNA-unwinding helicase and has been identified as one key player in the replication process of archea and eukarya. Despite the availability of considerable structural information on archeal MCMs, such information was missing for their C-terminal domain. In order to obtain more detailed structural information, we assigned the NMR chemical shifts for backbone and side chain nuclei for the MCM C-terminal winged helix domains of the archeal species Methanothermobacter thermautrophicus and Sulfolobus solfataricus.


MCM Replication Replicative helicase AAA+ ATPase Archea Winged helix NMR 



We are grateful to Dr. K.-H. Gührs for the mass spectrometric analyses of SsoE605. CW was supported by the Leibniz Graduate School on Ageing and Age-Related Diseases (LGSA). The FLI is a member of Science Association "Gottfried Wilhelm Leibniz (WGL)" and is financially supported by the Federal Government of Germany and the State of Thuringia.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Christoph Wiedemann
    • 1
    Email author
  • Oliver Ohlenschläger
    • 1
  • Barbara Medagli
    • 2
  • Silvia Onesti
    • 2
  • Matthias Görlach
    • 1
  1. 1.Biomolecular NMR SpectroscopyLeibniz Institute for Age Research—Fritz Lipman InstituteJenaGermany
  2. 2.Sincrotrone Trieste S.C.p.A.BasovizzaItaly

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