Backbone resonance assignments of the 42 kDa enzyme arginine kinase in the transition state analogue form
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Nearly complete backbone resonance assignments for the 357 residue, 42 kDa enzyme arginine kinase in a transition state analogue (TSA) complex are presented. The TSA is a quaternary complex of arginine kinase, MgADP, arginine, and nitrate. About 93 % (320 of 344) of the non-proline backbone amides were assigned using an enzyme enriched with 2H, 13C, and 15N in combination with three enzyme samples prepared with a single 15N-labeled amino acid (K, L, and R). The amide assignments will provide the foundation for investigating the dynamics of arginine kinase when in a TSA complex.
KeywordsArginine kinase Phosphagen kinase NMR spectroscopy Resonance assignment Enzyme dynamics Transition state analogue
This work was supported by the National Institutes of Health (GM77643).
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