1H, 13C, and 15N backbone and side chain resonance assignments of the C-terminal DNA binding and dimerization domain of v-Myc
The oncogenic transcription factor Myc is one of the most interesting members of the basic-helix-loop-helix-zipper (bHLHZip) protein family. Deregulation of Myc via gene amplification, chromosomal translocation or other mechanisms lead to tumorigenesis including Burkitt lymphoma, multiple myeloma, and many other malignancies. The oncogene myc is a highly potent transforming gene and capable to transform various cell types in vivo and in vitro. Its oncogenic activity initialized by deregulated expression leads to a shift of the equilibrium in the Myc/Max/Mad network towards Myc/Max complexes. The Myc/Max heterodimerization is a prerequisite for transcriptional functionality of Myc. Primarily, we are focusing on the apo-state of the C-terminal domain of v-Myc, the retroviral homolog of human c-Myc. Based on multi-dimensional NMR measurements v-Myc appears to be neither a fully structured nor a completely unstructured protein. The bHLHZip domain of v-Myc does not exist as a random coil but exhibits partially pre-formed α-helical regions in its apo-state. In order to elucidate the structural propensities of Myc in more detail, the backbone and side-chain assignments obtained here for apo-Myc are a crucial prerequisite for further NMR measurements.
KeywordsMyc Oncogene Transcription factor Random sampling Intrinsically disordered protein
The v-myc oncogene encoding the v-Myc protein is the transforming principle of avian Myelocytomatosis virus MC29 (Bister and Jansen 1986), an acute leukemia virus isolated from a chicken tumor (Ivanov et al. 1964). The viral genome encodes the transforming protein in form of a Gag-Myc hybrid protein encompassing 450 N-terminal residues encoded by the 5′ end of gag, linked to nine residues encoded by sequences derived from noncoding regions of c-myc (cellular myc), followed by 416 residues encoded by sequences derived from the protein coding regions of exons 2 and 3 of c-myc (Reddy et al. 1983; Bister and Jansen 1986). The v-Myc amino acid sequence differs from that of c-Myc by only a few amino acid substitutions. There are multiple mechanisms to drive c-myc into deregulated expression such as retroviral transduction, viral integration, chromosomal translocation, and gene amplification. Alteration of human c-myc via chromosomal translocation was found in Burkitt lymphoma (Dalla-Favera et al. 1982). Translocation of c-myc is also frequently seen in multiple myeloma cases (Shou et al. 2000). An enhanced TCF transcriptional activation of c-myc can be induced by defects in the Wnt-APC pathway (He et al. 1998). The transduced v-myc allele is one of the most potent transforming genes (Bister and Jansen 1986; Frykberg et al. 1987). It is capable to transform several different lineages of mammalian and avian cells. The most prominent fate for a cell after transformation by v-myc is enhanced proliferation. A v-myc transformed cell can become growth factor independent and as a consequence can develop an immortal and tumorigenic phenotype.
Myc’s importance and power over the fate of cells derives from the fact that Myc is a transcription factor. Together with its C-terminal binding partner Max (Myc associated factor X) it binds as a heterodimer to its cognate E-box DNA sequence (5′-CACGTG-3′) found frequently throughout the genome. A large number of target genes have been found. Currently the Myc database (myccancergene.org) lists more than 1,600 target genes. The Myc target genes are involved in many different functional processes including cell cycle control, cell growth, metabolism, apoptosis and immortality. The bHLHZip domain of Myc is essential for binding to Max and ranges from residue 314 to 416 of full-length v-Myc. Structural preformation of Myc in the apo-state (prior to Max binding) has already been shown via multi-dimensional NMR (Fieber et al. 2001). The partially folded monomeric Myc showed dynamic features such as helical fraying and a directed flexibility in the loop region.
Myc is thus another example for the growing evidence that binding interactions with intrinsically disordered proteins (IDP) largely proceed via conformational selection from a significantly narrowed conformation ensemble. In order to properly characterize the preformed structural ensemble of Myc, a complete signal assignment including side-chains is indispensible. Severe spectral overlaps make complete signal assignment a challenging task in IDPs.
Lavigne et al. provided 1998 a solution structure of N-terminal disulfide-linked versions of the c-Myc-Max heterodimeric Leucine Zipper solved by 2D H-NMR. A full backbone assignment of v-Myc in complex with its binding partner Max was published by Baminger et al. in 2004. Here we provide a complete signal assignment, backbone and side-chain, for the bHLHZip domain of apo (monomeric) v-Myc, using a set of high-dimensional triple resonance NMR experiments. Apo v-Myc shows a different spectra in respect to the heterodimeric Myc-Max complex. For more details compare with BMRB entry 6163. Our data will not only be crucial for the characterization of Myc’s apo-state, but also a valuable source of information for the analysis of amino-acid side chains in IDPs.
Methods and results
Expression and purification of v-Myc
The v-Myc bHLHZip domain encoding fragment was obtained by cleavage of the pET3dmycmax expression plasmid (Fieber et al. 2001; Baminger et al. 2004) using BamH1 and NcoI. The resulting fragment was ligated into the pETM-11 expression vector providing v-Myc with a N-terminal His-tag. The pETM-11v-myc plasmid was transformed into Rosetta(DE3)pLysS cells for protein expression following a protocol for efficient isotopic labeling of recombinant proteins using a fourfold cell concentration in minimal medium containing 15NH4Cl and 13C-glucose (Marley et al. 2001). v-Myc was expressed in inclusion bodies. The cells were collected after overnight expression at 30 °C by centrifugation at 4,000 rpm for 15 min and resuspended in 30 ml of ice-cold lysis buffer (20 mM NaH2PO4/Na2HPO4, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 6.5). The cells were lysed via sonication at 70 % for 3 × 5 min. After cell lysis, the lysate was cleared by centrifugation at 18,000 rpm for 20 min. The pellet was resuspended in 8 M Urea (including 1 mM EDTA, 100 mM NaCl and 20 mM imidazole, pH 8) and loaded onto a Ni2+-loaded HisTrap FF 5 ml affinity column (GE Healthcare) and eluted with 8 M Urea containing 100 mM imidazole. The eluate was collected and a step-wise refolding in 4 steps with buffer (20 mM NaH2PO4/Na2HPO4, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 6.5) was performed at 4 °C. After refolding the sample was concentrated to approximately 0.7 mM.
All spectra were acquired at 298 K on an Agilent Direct Drive 700 MHz spectrometer using a standard 5 mm 1H–13C–15N triple-resonance probehead.
The backbone 1H, 13C, and 15N resonances were assigned using sparse random sampling of indirectly detected time domains, in order to increase resolution. A 3D HNCO experiment was used as a base spectrum for SMFT (Sparse Multidimensional Fourier Transform) processing of higher dimensionality experiments (Kazimierczuk et al. 2010). Backbone assignment was achieved using 5D HN(CA)CONH (Kazimierczuk et al. 2010), (HACA)CON(CA)CONH, (H)NCO(NCA)CONH and HNCOCACB (Zawadzka-Kazimierczuk et al. 2012b) experiments. Side-chain assignments were obtained using 5D HabCabCONH (Kazimierczuk et al. 2010), and H(CC-tocsy)CONH (Kazimierczuk et al. 2009) experiments.
All NMR data sets were processed by multidimensional Fourier transformation using the home written software package (http://nmr700.chem.uw.edu.pl). The resonance assignment was performed using the TSAR program (Zawadzka-Kazimierczuk et al. 2012a). The input data for TSAR was prepared using Sparky software (Goddard and Kneller 2002).
Extent of assignment and data deposition
The assignments have been deposited in the BioMagResBank database (http://www.bmrb.wisc.edu) under accession number: 18580.
NMR experiments were carried out in the Structural Research Laboratory at the Faculty of Chemistry, University of Warsaw. This work was supported by the EAST-NMR project (contract no. 228461) inside of a transnational access program (proposal acronym: HISMYC). S. S., S. Ż, and W. K. thank the Foundation for Polish Science for support with the TEAM Programme. The TEAM programme is co-financed by the EU European Regional Development. We also thank the Austrian Science Fund (FWF): W1221-B03.
This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
- Frykberg L, Graf T, Vennstrom B (1987) The transforming activity of the chicken c-myc gene can be potentiated by mutations. Oncogene 1(4):415–422Google Scholar
- Goddard TD, Kneller DG (2002) Sparky 3. University of California, San FranciscoGoogle Scholar
- Ivanov X, Mladenov Z, Nedyalkov S, Todorov TG, Yakimov M (1964) Experimental investigations into avian leucoses. V. Transmission, haematology and morphology of avian myelocytomatosis. Bull Inst Pathol Comp Anim 10:5–38Google Scholar
- Zawadzka-Kazimierczuk A, Kozminski W, Billeter M (2012a) TSAR a program for automatic resonance assignment using cross-sections of high dimensionality, high-resolution spectra. J Biomol NMR 54:81–95 doi:10.1007/s10858-012-9652-3