Biomolecular NMR Assignments

, Volume 7, Issue 1, pp 47–49 | Cite as

NMR assignment of a PDZ domain in complex with a HPV51 E6 derived N-terminally pyroglutamic acid modified peptide

  • André Mischo
  • Oliver Ohlenschläger
  • Ramadurai Ramachandran
  • Matthias GörlachEmail author


The resonance assignment of an amino-terminal pyroglutamic acid containing peptide derived from the E6 protein of human papillomavirus (HPV) type 51 in complex with PDZ domain 2 of hDlg/SAP-97 is reported. The assignments include 1H, 13C and 15N resonances for the protein and peptide in the complex and all of the peptide’s pyroglutamic acid nuclei.


HPV E6 hDlg SAP-97 PDZ Pyroglutamic acid 



We acknowledge the funding of the Fritz Lipmann Institute by the State of Thuringia and the Federal Government of Germany. AM was funded by the Leibniz Graduate School on Ageing (LGSA). We thank S. Rothemund (IZKF Leipzig, Germany) for the synthesis of unlabelled peptides.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Charbonnier S, Nominé Y, Ramírez J, Luck K, Chapelle A, Stote RH, Travé G, Kieffer B, Atkinson RA (2011) The structural and dynamic response of MAGI-1 PDZ1 with noncanonical domain boundaries to the binding of human papillomavirus E6. J Mol Biol 406:745–763. doi: 10.1016/j.jmb.2011.01.015 CrossRefGoogle Scholar
  2. Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM (2010) Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127:2893–2917. doi: 10.1002/ijc.25516 CrossRefGoogle Scholar
  3. Keller R (2004) The computer aided resonance assignment tutorial. CANTINA Verlag, GoldauGoogle Scholar
  4. Liu Y, Henry GD, Hedge RS, Baleja JD (2007) Solution Structure of the hDlg/SAP97 PDZ2 domain and its mechanism of interaction with HPV-18 papillomavirus E6 protein. Biochemistry 46:10864–10874CrossRefGoogle Scholar
  5. Narisawa-Saito M, Kiyono T (2007) Basic mechanisms of high-risk human papillomavirus-induced carcinogenesis: roles of E6 and E7 proteins. Cancer Sci 98:1505–1511CrossRefGoogle Scholar
  6. Yugawa T, Kiyono T (2009) Molecular mechanisms of cervical carcinogenesis by high-risk human papillomaviruses: novel functions of E6 and E7 oncoproteins. Rev Med Virol 19:97–113CrossRefGoogle Scholar
  7. zur Hausen H (2009) Papillomaviruses in the causation of human cancers—a brief historical account. Virology 384:260–265CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • André Mischo
    • 1
  • Oliver Ohlenschläger
    • 1
  • Ramadurai Ramachandran
    • 1
  • Matthias Görlach
    • 1
    Email author
  1. 1.Leibniz-Institut für Altersforschung/Fritz-Lipmann-InstitutJenaGermany

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