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Biomolecular NMR Assignments

, Volume 6, Issue 2, pp 177–180 | Cite as

Backbone and side chain 1H, 13C, and 15N assignments of the ubiquitin-like domain of human HOIL-1L, an essential component of linear ubiquitin chain assembly complex

  • Yoshinori Uekusa
  • Syunsuke Mimura
  • Hiroaki Sasakawa
  • Eiji Kurimoto
  • Eri Sakata
  • Serve Olivier
  • Hirokazu Yagi
  • Fuminori Tokunaga
  • Kazuhiro Iwai
  • Koichi Kato
Article

Abstract

HOIL-1L and its binding partner, HOIL-1L interacting protein (HOIP), are essential components of linear ubiquitin (Ub) chain assembly complex (LUBAC), a 600-kDa enzyme complex catalyzing elongation of a tandemly connected Ub chain, which serve as a regulator of NF-κB activation. Specific interaction between the N-terminal Ub-like domain (UBL) of HOIL-1L and the Ub-associated domain (UBA) located at the central region of HOIP is shown to be involved in the formation of LUBAC. For better understanding of the mechanisms underlying the generation of the linear Ub chains by LUBAC, it is necessary to characterize the UBL-UBA interaction on the basis of structural data, which, however, is not available to date. Here we report backbone and side chain NMR assignments of the UBL of human HOIL-1L. By inspection of chemical shift index, it was predicted that HOIL-1L-UBL assumes a Ub fold followed by an α-helical segment, offering the basis for determination its 3D structure and interaction with HOIP-UBA in solution.

Keywords

HOIL-1L Ubiquitin-like domain NMR spectroscopy Resonance assignment 

Notes

Acknowledgments

We thank Kiyomi Senda and Kumiko Hattori (NCU) for their help in the preparation of recombinant proteins. This work was supported by Grants-in-Aid for Scientific Research and the Targeted Proteins Research Program.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Yoshinori Uekusa
    • 1
    • 2
  • Syunsuke Mimura
    • 2
  • Hiroaki Sasakawa
    • 1
    • 2
  • Eiji Kurimoto
    • 2
    • 3
  • Eri Sakata
    • 2
  • Serve Olivier
    • 1
    • 2
  • Hirokazu Yagi
    • 2
  • Fuminori Tokunaga
    • 4
    • 5
  • Kazuhiro Iwai
    • 4
  • Koichi Kato
    • 1
    • 2
  1. 1.Institute for Molecular Science and Okazaki Institute for Integrative BioscienceNational Institutes of Natural SciencesMyodaiji, OkazakiJapan
  2. 2.Graduate School of Pharmaceutical ScienceNagoya City UniversityMizuho-ku, NagoyaJapan
  3. 3.Faculty of PharmacyMeijo UniversityTempaku-ku, NagoyaJapan
  4. 4.Department of Biophysics and Biochemistry, Graduate School of MedicineOsaka UniversitySuita, OsakaJapan
  5. 5.Institute for Molecular and Cellular RegulationGunma UniversityMaebashi, GunmaJapan

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