Initiation of X-chromosome inactivation in female mammals depends on the non-coding RNA Xist. We have solved the NMR structure of a 14-nucleotide hairpin with a novel AUCG tetraloop fold from a Xist A-repeat that is essential for silencing. The 1H, 13C, 15N and 31P chemical shift assignments are reported.
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We thank Bernd Simon for help with NMR experiments and Frank Nelissen, Katia Zanier and Alexander Gasch for help with RNA sample preparation. We acknowledge the Bayerisches NMR Zentrum (BNMRZ) in Garching, the Biomolecular NMR Centre in Frankfurt and Wolfgang Bermel (Bruker, Karlsruhe) for spectrometer time. M.D. acknowledges support by an “E-STAR” Marie Curie Host fellowship for Early Stage Research Training funded by the EC’s FP6, contract MEST-CT-2004-504640. This work was supported by the EU STREP FSG-V-RNA, contract LSHG-CT-2004-503455 and the DFG-funded Cluster of Excellence “Center for integrated Protein Science, Munich” (CiPS-M). Initial work was supported by the EMBL Heidelberg.
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