Biomolecular NMR Assignments

, Volume 6, Issue 1, pp 75–77 | Cite as

1H, 13C, 15N and 31P chemical shift assignments of a human Xist RNA A-repeat tetraloop hairpin essential for X-chromosome inactivation

  • Malgorzata M. Duszczyk
  • Michael SattlerEmail author


Initiation of X-chromosome inactivation in female mammals depends on the non-coding RNA Xist. We have solved the NMR structure of a 14-nucleotide hairpin with a novel AUCG tetraloop fold from a Xist A-repeat that is essential for silencing. The 1H, 13C, 15N and 31P chemical shift assignments are reported.


NMR assignment Non-coding RNA RNA hairpin RNA tetraloop Xist RNA A-repeats X-inactivation 



We thank Bernd Simon for help with NMR experiments and Frank Nelissen, Katia Zanier and Alexander Gasch for help with RNA sample preparation. We acknowledge the Bayerisches NMR Zentrum (BNMRZ) in Garching, the Biomolecular NMR Centre in Frankfurt and Wolfgang Bermel (Bruker, Karlsruhe) for spectrometer time. M.D. acknowledges support by an “E-STAR” Marie Curie Host fellowship for Early Stage Research Training funded by the EC’s FP6, contract MEST-CT-2004-504640. This work was supported by the EU STREP FSG-V-RNA, contract LSHG-CT-2004-503455 and the DFG-funded Cluster of Excellence “Center for integrated Protein Science, Munich” (CiPS-M). Initial work was supported by the EMBL Heidelberg.

Supplementary material

12104_2011_9328_MOESM1_ESM.pdf (277 kb)
Supplementary material 1 (PDF 277 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Institute of Structural BiologyHelmholtz Zentrum MünchenNeuherbergGermany
  2. 2.Biomolecular NMR and Center for Integrated Protein Science Munich at Department ChemieTechnische Universität MünchenGarchingGermany

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