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1H, 13C and 15N chemical shift assignments of IPSEΔNLS

  • N. Helge Meyer
  • Gabriele Schramm
  • Michael Sattler
Article

Abstract

The interleukin-4-inducing principle from Schistosoma mansoni eggs (IPSE/alpha-1) is a major immunogenic component of schistosomes. It potently triggers the release of interleukin-4 from basophilic granulocytes in an IgE-dependent manner, suggesting a key function in the modulation of the host’s immune response to Schistosoma mansoni infection. Here we present the near complete assignment of an IPSE/alpha-1 variant, IPSEΔNLS, which comprises the core domain of the protein.

Keywords

NMR Schistosoma mansoni IgE 

References

  1. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6(3):277–293CrossRefGoogle Scholar
  2. Goddard T, Kneller D (2004) SPARKY 3. (www.cgl.ucsf.edu/home/sparky)
  3. Hotez PJ, Bethony JM, Diemert DJ, Pearson M, Loukas A (2010) Developing vaccines to combat hookworm infection and intestinal schistosomiasis. Nat Rev Microbiol 8(11):814–826. doi: 10.1038/nrmicro2438 CrossRefGoogle Scholar
  4. Mayerhofer H, Schramm G, Hatzopoulos GN, Mueller-Dieckmann C, Haas H, Mueller-Dieckmann J (2009) Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of interleukin-4-inducing principle from Schistosoma mansoni eggs (IPSE/alpha-1). Acta Crystallogr Sect F Struct Biol Cryst Commun 65(Pt 6):594–596. doi: 10.1107/S1744309109015899 CrossRefGoogle Scholar
  5. Sattler M, Schleucher J, Griesinger C (1999) Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Prog NMR Spectrosc 34:93–158CrossRefGoogle Scholar
  6. Schramm G, Falcone FH, Gronow A, Haisch K, Mamat U, Doenhoff MJ, Oliveira G, Galle J, Dahinden CA, Haas H (2003) Molecular characterization of an interleukin-4-inducing factor from Schistosoma mansoni eggs. J Biol Chem 278(20):18384–18392. doi: 10.1074/jbc.M300497200 CrossRefGoogle Scholar
  7. Schramm G, Mohrs K, Wodrich M, Doenhoff MJ, Pearce EJ, Haas H, Mohrs M (2007) Cutting edge: IPSE/alpha-1, a glycoprotein from Schistosoma mansoni eggs, induces IgE-dependent, antigen-independent IL-4 production by murine basophils in vivo. J Immunol 178(10):6023–6027Google Scholar
  8. Wilson MS, Mentink-Kane MM, Pesce JT, Ramalingam TR, Thompson R, Wynn TA (2007) Immunopathology of schistosomiasis. Immunol Cell Biol 85(2):148–154. doi: 10.1038/sj.icb.7100014 CrossRefGoogle Scholar
  9. Wistow G (1990) Evolution of a protein superfamily: relationships between vertebrate lens crystallins and microorganism dormancy proteins. J Mol Evol 30(2):140–145CrossRefGoogle Scholar
  10. Yamazaki T, Formankay JD, Kay LE (1993) 2-Dimensional NMR experiments for correlating C-13-beta and H-1-delta/epsilon chemical-shifts of aromatic residues in C-13-labeled proteins via scalar couplings. J Am Chem Soc 115(23):11054–11055CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • N. Helge Meyer
    • 1
    • 2
  • Gabriele Schramm
    • 3
  • Michael Sattler
    • 1
    • 2
  1. 1.Institute of Structural Biology, Helmholtz Zentrum MünchenNeuherbergGermany
  2. 2.Department Chemie, Biomolecular NMR and Center for Integrated Protein Science Munich (CiPSM)Technische Universität MünchenGarchingGermany
  3. 3.Cellular AllergologyResearch Center BorstelBorstelGermany

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