Biomolecular NMR Assignments

, Volume 5, Issue 2, pp 203–205 | Cite as

1H, 13C, and 15N resonance assignments for the CTD-interacting domain of Nrd1 bound to Ser5-phosphorylated CTD of RNA polymerase II

  • Karel KubíčekEmail author
  • Josef Pasulka
  • Hana Černá
  • Frank Löhr
  • Richard ŠteflEmail author


In this article, we report the resonance assignment of CTD-interacting domain (CID) of pre-mRNA down-regulation (Nrd)1 bound to Ser5-phosphorylated CTD (pSer5) of RNA Polymerase II. The presented assignment of backbone and side-chain resonances of the Nrd1 CID proton, carbon and nitrogen nuclei will allow studies of the structure and interaction of CID with carboxy-terminal domain (CTD) of the RNA polymerase II.


CTD-interacting domain (CID) Carboxy-terminal domain (CTD) RNA-processing Transcription termination 



This work was supported by HHMI/EMBO start-up grant, HFSP Career Development Award, Ministry of Education of the Czech Republic (MSM0021622413 and Ingo LA08008), GACR (204/08/1212 and 305/10/1490), and GAAV (IAA401630903). The NOESY spectra were obtained at the BMRZ NMR facility supported by the EU-NMR program (RII3-026145).


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.National Centre for Biomolecular Research, Central European Institute of Technology, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  2. 2.Institute of Biophysical Chemistry, Center for Biomolecular Magnetic ResonanceGoethe-UniversityFrankfurt am MainGermany

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