BRUNOL-3 protein, an alternate splicing factor, has been known for playing a major role in myotonic dystrophy. It binds to the cTNT m-RNA and prevents splicing of exon-5 region, leading to translation of troponin protein having differential affinity for Ca2+. Here, we report sequence-specific 1H, 13C, and 15N resonance assignments for RNA recognition motifs 1 and 2 of BRUNOL-3 protein.
BRUNOL-3 Myotonic dystrophy Cardiac troponin T Alternate splicing factor
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The work is funded by Department of Science and technology, Government of India under SERC FAST Track scheme vide diary no 100/IFD/5657/2009-2010. We thank Dr. Nicolas Charlet-Berguerand, IGBMC, University of Strasbourg, France for construct of BRUNOL-3. We thank Department of Biotechnology, Government of India for providing financial support for the High Field NMR spectrometers at the ICGEB, New Delhi and NII, New Delhi. We thank Prof. Virander S. Chauhan for his interest and support. Mr. Alok Sharma is acknowledged for his help during installation of the NMR at ICGEB. HB is a recipient of Council for Scientific and Industrial Research (CSIR) junior research fellowship. MK is a recipient of Indian Council for Medical Research (ICMR) junior research fellowship.
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