Biomolecular NMR Assignments

, Volume 4, Issue 1, pp 29–32 | Cite as

Backbone and ILV side chain methyl group assignments of the integral human membrane protein VDAC-1

  • Sebastian Hiller
  • Thomas J. Malia
  • Robert G. Garces
  • Vladislav Y. Orekhov
  • Gerhard Wagner


The voltage dependent anion channel (VDAC) forms a channel for metabolites and nutrients in the outer membrane of mitochondria, and it is also involved in apoptotic pathways. Here, we report sequence-specific NMR assignments for the isoform 1 of human VDAC reconstituted in lauryldimethylamine oxide (LDAO) detergent micelles. The assignments were deposited in the BMRB data base with accession number 16381.


Voltage dependent anion channel Voltage gating β-Barrel Protein refolding Membrane protein structure Multi-dimensional decomposition (MDD) TROSY Selective labeling 4D-NOESYs Non-uniform sampling (NUS) 



This work was supported by the United States National Institute of Health by the roadmap grant GM075879 and the grants GM066360, GM47467 and EB 002026 for purchase, operation and maintenance of the instruments. S.H. was supported in part by the Swiss National Science Foundation.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Sebastian Hiller
    • 1
    • 2
  • Thomas J. Malia
    • 1
    • 4
  • Robert G. Garces
    • 1
  • Vladislav Y. Orekhov
    • 1
    • 3
  • Gerhard Wagner
    • 1
  1. 1.Department of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonUSA
  2. 2.Laboratory of Physical ChemistryZurichSwitzerland
  3. 3.Swedish NMR CentreUniversity of GothenborgGothenborgSweden
  4. 4.Centocor, Inc.RadnorUSA

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