Biomolecular NMR Assignments

, Volume 4, Issue 1, pp 29–32 | Cite as

Backbone and ILV side chain methyl group assignments of the integral human membrane protein VDAC-1

  • Sebastian Hiller
  • Thomas J. Malia
  • Robert G. Garces
  • Vladislav Y. Orekhov
  • Gerhard Wagner
Article

Abstract

The voltage dependent anion channel (VDAC) forms a channel for metabolites and nutrients in the outer membrane of mitochondria, and it is also involved in apoptotic pathways. Here, we report sequence-specific NMR assignments for the isoform 1 of human VDAC reconstituted in lauryldimethylamine oxide (LDAO) detergent micelles. The assignments were deposited in the BMRB data base with accession number 16381.

Keywords

Voltage dependent anion channel Voltage gating β-Barrel Protein refolding Membrane protein structure Multi-dimensional decomposition (MDD) TROSY Selective labeling 4D-NOESYs Non-uniform sampling (NUS) 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Sebastian Hiller
    • 1
    • 2
  • Thomas J. Malia
    • 1
    • 4
  • Robert G. Garces
    • 1
  • Vladislav Y. Orekhov
    • 1
    • 3
  • Gerhard Wagner
    • 1
  1. 1.Department of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonUSA
  2. 2.Laboratory of Physical ChemistryZurichSwitzerland
  3. 3.Swedish NMR CentreUniversity of GothenborgGothenborgSweden
  4. 4.Centocor, Inc.RadnorUSA

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