1H, 13C, and 15N NMR assignments for the Bacillus subtilis yndB START domain
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The steroidogenic acute regulatory-related lipid transfer (START) domain is found in both eukaryotes and prokaryotes, with putative functions including signal transduction, transcriptional regulation, GTPase activation and thioester hydrolysis. Here we report the near complete 1H, 15N and 13C backbone and side chain NMR resonance assignments for the Bacillus subtilis START domain protein yndB.
KeywordsBacillus subtilis Protein yndB START domain Lipid binding NMR Resonance assignments
We thank Prof. Diana Murray for stimulating discussions regarding the NESG START domain project and Dr. John Cort at PNNL for providing the 750 MHz NMR data. This work was supported by a grant from the Protein Structure Initiative of the National Institutes of Health (U54 GM074958 to G.T.M.), Nebraska Tobacco Settlement Biomedical Research Development Fund and by the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences. Research was performed in facilities renovated with support from the NIH under Grant RR015468-01. NMR spectra were acquired at the US Department of Energy Office of Biological and Environmental Research) located at Pacific Northwest National Laboratory and operated for DOE by Battelle (contract KP130103).
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